Isolation and characterization of microsatellite loci in the endangered freshwater fish <Emphasis Type="Italic">Varicorhinus alticorpus</Emphasis> (Cyprinidae) |
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Authors: | Tzen-Yuh Chiang Teh-Wang Lee Feng-Jiau Lin Kuo-Hua Huang Hung-Du Lin |
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Institution: | (1) Department of Life Sciences, National Cheng Kung University, Tainan, 701, Taiwan;(2) Taiwan Endemic Species Research Institute, Jiji, 552, Taiwan;(3) Department of Material Science, National University of Tainan, Tainan, Taiwan |
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Abstract: | Varicorhinus alticorpus (Cyprinidae) is an endangered freshwater fish with a scattered distribution in the southern and eastern regions of Taiwan.
We described the development of nine polymorphic microsatellite loci in V. alticorpus for genetic studies. These new markers were tested in 20 individuals of the rare species. The number of alleles ranged from
4 to 13. Expected (H
E) and observed (H
O) heterozygosities ranged from 0.692 to 0.892 (averaged at 0.821) and from 0.000 to 0.350 (averaged at 0.088), respectively.
All loci are significantly deviated from Hardy–Weinberg expectations due to the heterozygote deficiency. This suite of highly
polymorphic microsatellites provides the first chance to undertake a conservation program for this species in Taiwan. It is
essential to evaluate its genetic diversity and the fine-scale population structure.
Tzen-Yuh Chiang, Teh-Wang Lee, and Feng-Jiau Lin contributed equally to this work. |
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Keywords: | Microsatellite PIMA RAPD-PCR enrichment Varicorhinus alticorpus |
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