褐藻裙带菜色素蛋白复合物的性质*

用去污剂DMG增溶褐藻裙带菜(Undaria pinnatifida)的类囊体膜,通过PAGE分离色素-蛋白复合物并分析其性质,结果表明:CPⅠa和CPⅠ都含有66kDa的多肽,低温荧光发射光谱中有715nm的长波荧光峰,激发光谱测定结果表明CPⅠa是含有墨角藻黄素的叶绿素a/c-蛋白复合物,CPⅠ是只含有叶绿素a的色素-蛋白复合物。CPa含有51、37、34和20kDa四种多肽,低温荧光发射峰位于683nm,激发光谱表明它含有叶绿素a、c和少量墨角藻黄素,是裙带菜的PSⅠ复合物。其余5条为捕光色素-蛋白复合物,它们都是由20kDa的多肽组成,其中LHC₁和LHC₃有相似的光谱特性,是墨角藻黄素-叶绿素a/c-蛋白复合物,LHC₂、LHC₄和LHC₅的光谱特性相似,是叶绿素a/c-蛋白复合物。

The characteristics of pigment-protein complexes from brown alga Undaria pinnatifida

When the thylakoid membranes of brown alga Undaria pinnatifida were solubilized with DMG and subjected to PAGE, eight pigment-protein complexes were resolved.Both CP Ⅰ a and CP Ⅰ contain a polypeptide of 66 kDa,and they have a long- wavelength fluorescence emission peak at 715 nm at 77 K,the excitation spectra shows CP Ⅰ a is fucoxanthin-chla/c-protein complex and CP Ⅰ is chla-protein complex.CPa contains 51,37,34 and 20 kDa polypeptides,its fluorescence emission maximum at 77 K is at 683 nm,the excitation spectra shows that CPa is a fucoxanthin-chla/c-protein complex.The other five complexes are related to LHC,they all present a major polypeptide component of 20 kDa,the spectra characteristics of LHC₁ and LHC₃ are similar,they are all fucoxanthin-chla/c-protein complexes,LHC₂,LHC₄ and LHC₅ are chla/c-protein complexes.