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草鱼外周血细胞数量及其在胁迫或感染下的变化
引用本文:张妍,张其中,罗新,崔淼.草鱼外周血细胞数量及其在胁迫或感染下的变化[J].生态科学,2012(3):295-300.
作者姓名:张妍  张其中  罗新  崔淼
作者单位:暨南大学水生生物研究所
基金项目:国家高技术研究发展计划(“863”计划)项目(2011AA10A216-2);国家自然科学基金项目(40976066,31170474);广东省海洋与渔业局科技推广项目(A201001HO1)
摘    要:为了提供可反映草鱼(Ctenopharyngodon idellus)健康状况的血细胞指标的基础数据,研究了4种质量规格组的正常草鱼的外周单位体积血细胞数量,以及分别在硫酸铜或敌百虫处理下和多子小瓜虫感染后的变化。结果表明,1 000 g组草鱼的单位体积红细胞数显著高于其他3种质量规格组;60 g组草鱼的单位体积白细胞数显著低于其他3种质量规格组。硫酸铜或敌百虫处理组的单位体积红、白细胞数显著高于对照组;多子小瓜虫感染组的单位体积白细胞数极显著高于对照组。这里提出一个初步参考指标,60~1 000 g健康草鱼的红细胞值为1.15×106~3.33×106cells/μL,白细胞值为2.20×105~4.40×105cells/μL。

关 键 词:草鱼  外周血细胞  硫酸铜  敌百虫  多子小瓜虫

Quantity and its change of peripheral blood cells of stressed or infected grass carp
ZHANG Yan,ZHANG Qi-zhong,LUO Xin,CUI Miao.Quantity and its change of peripheral blood cells of stressed or infected grass carp[J].Ecologic Science,2012(3):295-300.
Authors:ZHANG Yan  ZHANG Qi-zhong  LUO Xin  CUI Miao
Institution:Institute of Hydrobiology,Jinan University,Guangzhou 510632,China
Abstract:Erythrocyte and leucocyte number per microlitre was investigated for each blood sample of the fish in the test and control group.The results showed that the erythrocyte number per microlitre in the fish of 1 000 g group was significantly higher than that of the other three groups with different fish sizes.The leucocyte number per microlitre in the fish of 60 g group was significantly lower than that of other three groups with different fish sizes.The leucocyte and erythrocyte number per microlitre of copper sulfate or trichlorfon treated group was significantly higher than that of control group.The leucocyte number per microlitre of grass carps infected by I.multifiliis was significantly higher than that of control group.The results suggested that the erythrocytes were 1.15×106-3.33×106 cells/μL and the leucocytes were 2.20×105-4.40×105cells/μL in the healthy grass carps weighing between 60 g and 1 000 g.
Keywords:Ctenopharyngodon idellus  peripheral blood cells  copper Sulfate  trichlorfon  Ichthyophthirius multifiliis
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