利用PCR方法研究广州市景观湖产毒微囊藻的季节分布特点

为研究景观水体中产毒微囊藻的季节性分布特点,利用针对蓝藻和微囊藻的16 srDNA、微囊藻毒素合成酶mcyB 基因的部分核苷酸序列设计和筛选的特异性引物,对广州市内8个景观湖108份水样进行了冬季、夏季和秋季的二重及套式PCR的检测.结果显示,在冬季能被检测出的产毒微囊藻的阳性水样为42份,夏季为102份,而秋季为100份;阳性率分别为38.9%、94.4%、92.5%,产毒微囊藻在夏季和秋季阳性率高.结果表明在冬季、夏季和秋季均有产毒微囊藻分布;夏、秋季是广州市景观水体微囊藻污染的高峰季节,值得引起水文部门足够的重视.

Using PCR for studying seasonal distribution of toxic microcystis from scenic sites in Guangzhou

Base on the conserved 16S rDNA gene of microcystis, cyanobacteria and microcystin synthetase gene B (mcyB). A duplex PCR and nested PCR were used to detect toxic species at eight scenic sites, including 108 water samples in Guangzhou of Guangdong province in three seasons. Results show that total positive ratio of toxic Microcystis was 38.9% in winter, 94.4% in summer, and 92.5% in autumn. This study demonstrated that toxic Microcystis distributed in winter, summer and autumn and Microcystins pollutions in Guangzhou scenic sites was mainly in summer and autumn.