温度光照pH对雨生血球藻CG-11绿色营养细胞生长的影响

采用BBM培养基,以雨生血球藻Haematococcus pluvialis CG-11为研究材料,进行环境因子对雨生血球藻绿色营养细胞生长影响的实验。温度梯度为16℃、20℃、24℃、28℃;光照梯度为30μmoL photons m^-2·s^-1、60μmoL photons m^-2·s^-1、90μmoL photons m^-2·s^-1、120μmoL photons m^-2·s^-1。初始pH值为6.0、7.0、8.0、9.0,进行单因子试验,测定各组的藻密度及色素含量。结果显示,雨生血球藻H.pluvialis CG-11生长的最适温度范围20～24℃,光照90μmol photons m^-2·s^-1,初始pH8.0。

Studies on temperature,illumination and pH of green vegetative cell of Haematococcus pluvialis CG-11

An economic microalgae Haemalococcus pluvialis CG-11. was used as experimental materia1, and was cultivated in the BBM medium. The influence of the physical factors(temperature, illumination and pH)on growth, chlorophylla content, carotenoid content, dry weight and astaxanthin content of the green vegetative cell was studied. These experiments were carried out separately as three single factor tests when four temperatures (16℃, 20℃, 24℃ and 28℃), four illuminations (30 μmol photons m^-2·s^-1, 60 μmol photons m^-2·s^-1, 90 μmol photons m^-2·s^-1, 120 μmol photons m^-2·s^-1) and four pH (6.0, 7.0, 8.0, 9.0) were adapted. The results showed that H. pluvialis CG-11. grew well in the range of 20～24℃. The optimum light intensity was about 90 μmoL photons m^-2·s^-1, and neutral or alkalescent environment was suitable to H. pluvialis CG-11. growth at pH 8.0.