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克氏原螯虾(Procambarus clarkii)血细胞染色方法的研究
引用本文:周晖,谢数涛,龙思思,段舜山.克氏原螯虾(Procambarus clarkii)血细胞染色方法的研究[J].生态科学,2003,22(2):130-132.
作者姓名:周晖  谢数涛  龙思思  段舜山
作者单位:暨南大学水生生物研究所,广州,510632
摘    要:甲壳类血细胞的形态和分类是甲壳类免疫学研究的基础。本文选择三种甲壳类血细胞的常用染料:瑞氏染液、姬姆萨染液和瑞氏—姬姆萨混合染液,研究它们对于克氏原螯虾血细胞的染色效果。通过改变染色时间、染色温度、分色方式以及分色时间等染色条件,观察、比较不同染色条件下血细胞内的颗粒、细胞核和细胞质的着色情况,以及细胞整体轮廓清晰程度,确定适用于克氏原螯虾血细胞染色的理想染色方法,并建立相应的操作程序。

关 键 词:克氏原螯虾  血细胞  染色  甲壳类  免疫学
文章编号:1008-8873(2003)02-130-03
修稿时间:2003年3月20日

The studies of staining protocols of haemocytes from clawfish Procambarus clarkii
ZHOU hui,XIE Shu-tao,LONG Si-si DUAN Shun-shan.The studies of staining protocols of haemocytes from clawfish Procambarus clarkii[J].Ecologic Science,2003,22(2):130-132.
Authors:ZHOU hui  XIE Shu-tao  LONG Si-si DUAN Shun-shan
Abstract:The morphology and the classification of haemocytes are the foundations of the immune system studies in Crustracean.Dyeing results of Giemsa,Wright's,Wrighf s-Giemsa by different staining protocols were studied.Using different concentrations and dosages of dyes,we compared the dyeing results of haemocytes with different staining time,staining temperature,decoloration time and methods.The appropriate protocols of three staining methods were determined according to the dyeing results of granules,nuclei and cytoplasms.
Keywords:Procambarus clarkii  Haemocytes  Staining
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