环境因子对眼斑拟石首鱼精子活力的影响

通过测定精子的激活率、运动时间及寿命,研究了pH、盐度、离子及葡萄糖等因子变化对眼斑拟石首鱼(Csiaenops ocellatus)精子活力的影响.结果表明,眼斑拟石首鱼精液中精子平均浓度为(1.203±0.22)×10~(10)个·mL~(-1);精子激活与运动的适宜盐度范围为20～35,其中盐度25时精子的激活率、运动时间及寿命分别达94.32%、9.14min及12.55min;精子激活与运动的适宜pH为6.0～8.5,适宜的KCl、NaCl及CaCl_2溶液浓度分别为600～700 mmol·L~(-1)、600mmol·L~(-1)及400～600 mmol·L~(-1),适宜的葡萄糖溶液浓度为700～900mmol·L~(-1);精子在缺少HCO_3~-或Mg~(2+)或Ca~(2+)的人工海水中激活率与在人工海水中的激活率无显著差异,但运动时间和寿命有所缩短.

Effects of environmental factors on the vitality of sperm in csiaenops ocellatus

The effects of pH value, salinity, glucose and ions solutions on the sperm vitality of Csiaenops ocellatus were investigated by measuring the activation ration, moving time and life-span of sperm. The results indicated that the average density of sperm was (1.203±0.22) ×10~(10)·ml~(-1) and the suitable salinity for sperm activation and movement was 20～35, while pH value 6.0～8.5. The activation rate, moving time and life-ban of sperm reached to 94.32%, 9.14 min and 12.55 min respectively when the sperm was activated in salinity 25. The proper concentration of KCl, NaCl, CaCl_2 solutions for sperm activation and movement was 600～700 mmol·L~(-1),600 mmol·L~(-1) and 400～600 mmol·L~(-1) respectively, while the proper concentration of glucose solution was 700～900mmol·L~(-1). No significant differences were found in the activation rate when the sperm was activated by the artificial seawater lacking of HCO_3~-,Mg~(2+) or Ca~(2+), but the moving time and survival time of sperm became shorter.