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注射光合细菌后罗氏沼虾血细胞组成的变化
引用本文:龙思思,谢数涛,周晖,骆育敏.注射光合细菌后罗氏沼虾血细胞组成的变化[J].生态科学,2003,22(2):124-126.
作者姓名:龙思思  谢数涛  周晖  骆育敏
作者单位:暨南大学水生生物研究所,广州,510632
摘    要:以光合细菌作为刺激物,对罗氏沼虾进行肌肉注射,在注射后12、24、36、48、60h依次从罗氏沼虾心脏取血,用改良后的瑞氏染色法制成血涂片,光镜观察罗氏沼虾血细胞组成的变化。结果发现罗氏沼虾透明细胞在循环血细胞中所占地比例由正常状态下的66.62%略微下降至60%左右;36h左右所占比例急剧下降到30%左右;48h左右开始逐步回升;60h恢复至55%;接受刺激后的变化遵循缓慢下降、急剧下降、缓慢回升的过程。

关 键 词:光合细菌  罗氏沼虾  血细胞  免疫学
文章编号:1008-8873(2003)02-124-03
修稿时间:2003年5月2日

The Change in constitution of Macrobrachium rosenbergii hemocyte after injection of PSB
LONG Si-si,XIE Shu-tao,ZHOU Hui,LUO Yu-min.The Change in constitution of Macrobrachium rosenbergii hemocyte after injection of PSB[J].Ecologic Science,2003,22(2):124-126.
Authors:LONG Si-si  XIE Shu-tao  ZHOU Hui  LUO Yu-min
Abstract:The change in the constitution of Macrobrachium rosenbergii hemocytes was observerd and the functions of different type hemocytes were speculated after PSB into the muscle of Macrobrachium rosenbergii as a exciter.Stained by Wright's staining,the staining slides were observed under the light microscope at 12,24,36,48,60 hours after received injections.It shows that hyaline cells normally constituted 66.62% of the total amount of hemocytes.In 24 hours after received injection,the ratio of hyaline cells to the total amount of hemocytes became to about 60%,after 36 hours,it declined sharply to nearly 30%,after 48 hours,it began to rise,and after 60 hours it recovered to 55%.The process of change is that the ratio declined slowly at first,then to declined sharply,and to recovered later.
Keywords:Macrobrachium rosenbergii  PSB  Hemocyte
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