太湖蓝藻滤液的遗传毒性研究

蓝藻爆发是环境污染引发的重要事件之一,随之产生的蓝藻毒素又直接危及区域水安全.该论文采用蚕豆和大蒜根尖微核试验研究了太湖蓝藻暴发期间蓝藻滤液的遗传毒性.结果表明,同阴性对照相比,所有试验处理对蚕豆根尖细胞微核发生率的影响显著增加;对大蒜根尖细胞微核发生率而言除蓝藻滤液8倍稀释液的影响不显著外,其它水平效应显著高于阴性对照,而且表现出一定的剂量效应.暴发期蓝藻滤液原液对蚕豆根尖细胞微核发生率影响显著高于阳性对照(0.8mg·mL^-1环磷酰胺)的效应,从而说明蓝藻暴发时期蓝藻滤液具有较强的遗传毒性.通过微核试验效果分析,蚕豆作为植物监测系统的敏感性和稳定性都优于大蒜材料.

The study on genotoxicity of blue-green algae filtrate during blue-green algae fast growth in Lake Tai

The genetic toxicology of blue-green algae filtrate of Tai Lake was investigated using micronucleus test of root tip cell of Vicia faba and Allium sativum L..The results showed that in contrast to negative control(pure water),the effects of other treatments on micronucleus(MCN) of two plant root cells were significantly increased except for those of eight times diluent of blue-green algae filtrate,and that the effects which induced micronucleus in root-tip cells of plant exhibited dose-effect.The effect of stock solution of blue-green algae filtrate on MCN of root tip cell of broad bean was significantly higher than that of positive control(0.8 mg·mL^-1 cyclophosphamide).It can be concluded that there is strong genotoxicity in blue-green algae filtrate during blue-green algae fast growth in Lake Tai.