| 近江牡蛎HSP70基因对溶藻弧菌感染的反应 |
| |
| 引用本文： | 陈晨,张其中,张占会,崔淼.近江牡蛎HSP70基因对溶藻弧菌感染的反应[J].生态科学,2011,30(3):295-300. |
| |
| 作者姓名： | 陈晨 张其中 张占会 崔淼 |
| |
| 作者单位： | 暨南大学水生生物研究所,热带亚热带水生态工程教育部工程研究中心,广东省高校水体富营养化与赤潮防治重点实验室,广州,510632 |
| |
| 基金项目： | 国家自然科学基金项目，暨南大学创新基金 |
| |
| 摘 要： | 采用实时荧光定量RT-PCR方法,检测了注射溶藻弧菌(Vibiro alginolyticus)后近江牡蛎鳃,闭壳肌,消化腺,外套膜,心脏以及血细胞中HSP70基因的表达变化。结果显示近江牡蛎这五种器官组织中的HSP70基因表达量均出现显著性高表达,且在鳃、外套膜和血细胞中的HSP70基因表达变化规律表现为典型的时间依赖性。血细胞中,显著高表达的峰值出现在24h,至72h恢复到对照水平,高表达持续时间最长:鳃中表达峰值出现时间较早,在第3h,随后在第12h便恢复到对照水平;外套膜,消化腺以及心脏中的峰值分别出现在6h,6h和3h,而在闭壳肌组织中,没出现显著性高表达。由此可见,近江牡蛎HSP70s可能在机体抗菌免疫过程中起了重要作用。
|
| 关 键 词： | 近江牡蛎 HSP70基因 溶藻弧菌 实时荧光定量RT-PCR mRNA表达 |
mRNA Expression of Heat Shock protein 70 gene in the oyster(Crassostrea hongkongensis)responding to Vibiro alginolyticus challenge |
| |
| CHEN Chen,ZHANG Qi-zhong,ZHANG Zhan-hui,CUI Miao.mRNA Expression of Heat Shock protein 70 gene in the oyster(Crassostrea hongkongensis)responding to Vibiro alginolyticus challenge[J].Ecologic Science,2011,30(3):295-300. |
| |
| Authors: | CHEN Chen ZHANG Qi-zhong ZHANG Zhan-hui CUI Miao |
| |
| Institution: | CHEN Chen,ZHANG Qi-zhong~*,ZHANG Zhan-hui,CUI Miao Institute of Hydrobiology,Jinan University,Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering Ministry of Education,Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institutes,Guangzhou 510632,China |
| |
| Abstract: | In order to investigate the role of HSP70 of oyster(Crassostrea hongkongensis) against bacterial infection,we established a real-time,quantitative fluorescent RT-PCR method to examine the expression patterns of HSP70 mRNA in mantle,digestive gland,gills,muscle,heart and haemocytes of the oyster responding to Vibiro alginolyticus challenge.The results showed that after the oysters were injected with live V.alginolyticus,HSP70 mRNA expression in gills,mantle and haemocytes was up-regulated at first,peaked at ... |
| |
| Keywords: | Crassostrea hongkongensis HSP70 gene Vibiro alginolyticus real-time fluorescence quantitative RT-PCR mRNA expression |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
