不同无机碳源对粉核油球藻生长的影响

研究了2种无机碳源对粉核油球藻(Pinguiococcus pyrenoidosus CCMP2078)生长的影响。结果表明,适量添加NaHCO₃和通CO₂对P.pyrenoidosus CCMP2078的生长均有促进作用,NaHCO₃的添加方式对该藻的生长具有显著差异。一次性添加和分批添加NaHCO₃的优化浓度分别为5mmoL·L^-1和20mmoL·L^-1,后者对数生长末期的细胞密度、干重和比生长速率均最高,分别为8.93×10⁶cells·mL^-1,0.247g·L^-1和0.225dL^-1。通入CO₂的体积分数为0.5%时,最有利于藻细胞的生长,对数生长末期的细胞密度、干重和比生长率分别为3.83×10⁷cells·mL^-1、0.42g·L^-1和0.212d^-1。

Effects of inorganic carbon sources on growth of Pinguiococcus pyrenoidosus CCMP 2078

Effects of two types of inorganic carbon sources on the growth of Pinguiococcus pyrenoidosus CCMP 2078 were investigated in this study. The results showed that the growth of P.pyrenoidosus CCMP 2078 was significantly promoted by adding appropriate amount of NaHCO₃ or CO₂, and there were significant differences among different additions of NaHCO₃. The optimal concentrations of NaHCO₃ added for one time and added equally for several times were 5 mmoL·L^-1 and 20 mmoL·L^-1, respectively. For the latter treatment, parameters of cell density, dry weight, and the specific growth rate reached to the highest value, which were 8.93×10⁶cells·mL^-1, 0.247 g·L^-1, and 0.225 d^-1, respectively. When CO₂ content was 0.5%, P. pyrenoidosus CCMP 2078 reached to the highest growth rate, and parameters of cell density, dry weight, and the specific growth rate were 3.83×10⁷cells·mL^-1, 0.42 g·L^-1, and 0.212 d^-1, respectively.