| 沙田柚茎尖嫁接苗离体培养的研究 |
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| 引用本文： | 董高峰,黄涛,李耿光,张兰英.沙田柚茎尖嫁接苗离体培养的研究[J].生态科学,2001,20(3):14-19. |
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| 作者姓名： | 董高峰 黄涛 李耿光 张兰英 |
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| 作者单位： | 中国科学院华南植物研究所, |
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| 基金项目： | 国家自然科学基金资助项目(39870539);广东省自然科学基金资助项目(980478);广东省创新百项工程资助项目(99) |
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| 摘 要： | 对沙田柚茎尖嫁接苗的离体培养进行了研究,结果表明:顶芽在MS 0.5mg/L6-BA上生长较好,成活率为100%,单个外植体平均不定芽数为2.2,但不定芽生长极慢,成苗困难.加入0.2～0.5mg/LIBA,顶芽生长加快,但单个外植体平均不定芽数下降.带有1cm长枳壳砧木的茎尖在MS 0.5mg/L6-BA上可以形成丛芽,单个外植体平均不定芽数为4.5,而且不定芽生长迅速.虽然我们未能诱导外植体不定根发生,但通过试管嫁接可以获得完整植株.
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| 关 键 词： | 沙田柚 茎尖嫁接苗 离体培养 不定芽 |
| 文章编号： | 1008-8873(2001)03-0014-06 |
| 修稿时间： | 2001年6月18日 |
Studies on the Culture of Shoot_tip Grafting Plantlets of Shation Pomelo in Vitro |
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| DONG Gao-feng,HUANC Tao,LI Geng-guang,ZHAJVG Lan-ying.Studies on the Culture of Shoot_tip Grafting Plantlets of Shation Pomelo in Vitro[J].Ecologic Science,2001,20(3):14-19. |
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| Authors: | DONG Gao-feng HUANC Tao LI Geng-guang ZHAJVG Lan-ying |
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| Abstract: | The culture of shoot-tip grafting plantlets of Shatian pomelo had been studied in vita. The results showed the shoot-tips growed better in MS 6-BA 0.5 mg/L, the survival percentage got100%, and the average adventitious shoots per explant were 2.2, but the average adventitious shoots growed slowly, they were very difficult to form full plantlets. The shoot-tips growed rapidly if we added 0.2 .5 mg/ IBA into MS 6 BA 0.5 mg/L, but the average adventitious shoots per explant decreased. The shoot tips of Shatian pommelo with 1 cm stock of citrange could form the clump-shoots, their survival percentage also got 100%, the average adventitious shoots per explant were 4.5, and the average adventitious shoots cauld grow rapidly. We could acquire the full plantlets via the tube-grafting although we didn?t induce the generation of adventitious mats. |
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| Keywords: | Citrus grandis Osbeck (Shatian pomelo) shoot-tip grafting plantlet culture in vitro adventitious shoot |
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