Molecular cloning and mRNA expression profiling of the first specific jasmonate biosynthetic pathway gene allene oxide synthase from <Emphasis Type="Italic">Hyoscyamus niger</Emphasis> |
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Authors: | K?Jiang Y?Pi Z?Huang R?Hou Zh?Zhang J?Lin X?Sun Email author" target="_blank">K?TangEmail author |
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Institution: | (1) State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Morgan-Tan International Center for Life Sciences, Fudan University, Shanghai, 200433, China;(2) Plant Biotechnology Research Center, School of Agriculture and Biology, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Institute of Systems Biology, Shanghai Jiao Tong University, Shanghai, 200030, China |
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Abstract: | In the endeavor to enhance the production of pharmaceutically valuable tropane alkaloids including hyoscyamine and scopolamine
in Hyoscyamus niger, methyl jasmonate (MeJA) showed significant stimulation both in tropane biosynthetic pathway enzymes activities and tropane
alkaloids yields. Therefore it was speculated that genetic engineering of jasmonate biosynthetic pathway might enhance the
endogenous jasmonates concentration, followed by stimulating the production of tropane alkaloids. Herein a full-length cDNA
encoding allene oxide synthase (AOS, EC 4.2.1.92), the first committed step enzyme in jasmonate biosynthetic pathway was reported
(named HnAOS, GenBank accession: EF532599). HnAOS was a novel member of the cytochrome P450 (CYP74A) subfamily. Real-time quantitative PCR analysis showed that HnAOS mRNA accumulated mainly in stems, and responded significantly to wounding or methyl jasmonate.
The article is published in the original. |
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