首页 | 本学科首页   官方微博 | 高级检索  
   检索      


In vitro differentiation of human dental follicle cells with dexamethasone and insulin
Authors:Morsczeck C  Moehl C  Götz W  Heredia A  Schäffer T E  Eckstein N  Sippel C  Hoffmann K H
Institution:Stiftung caesar, Center of Advanced European Studies and Research, Ludwig Erhard Allee 2, 53175 Bonn, Germany. morsczeck@gmx.de
Abstract:The dental follicle is an ectomesenchymally derived connective tissue harboring precursor cells for the tooth supporting apparatus. In this study, we examined gene expression of freshly isolated human dental follicle cells during osteogenic differentiation in vitro. These plastic adherent fibroblastic cells express Notch-1, nestin and vimentin. We differentiated dental follicle cells with dexamethasone or insulin-based protocols into membrane-like structures containing mineralizing foci. An analysis of mineralized tissue with atomic force microscopy illustrated a bone and cementum-like structure. A real-time RT-PCR analysis was developed to investigate expression of typical osteoblast or cementoblast related genes during differentiation. Gene expressions of osteocalcin (OCN), bone morphogenic protein (BMP)-2 and nestin were increased during the both differentiation approaches. Our work demonstrates differentiation of dental follicle cells with an insulin-based protocol for the first time.
Keywords:Real‐time RT‐PCR  Dental follicle  Differentiation  Dexamethasone  Insulin
本文献已被 PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号