Structural and functional diversity among bacterial interspersed mosaic elements (BIMEs) |
| |
| Authors: | Sophie Bachellier William Saurin David Perrin Maurice Hofnung Eric Gilson† |
| |
| Institution: | Unitéde Programmation Moléculaire et Toxicologie Génétique, CNRS URA 1444, Institut Pasteur, 25 rue du Docteur Roux, Paris 75015, France. |
| |
| Abstract: | Palindromic units (PU or REP) were defined as 40-nucleotide DNA sequences which are highly repeated in the genome of several members of the Enterobacteriaceae. They were shown to be a constituent of the bacterial interspersed mosaic element (BIME), in which they are associated with other repetitive sequences. We report here that Escherichia coli PU sequences contain three motifs (Y, Z1 and Z2), leading to the definition of two BIME families. The BIME-1 family, highly conserved over 145 nucleotides, contains two PUs (motifs Y and Z1). The BIME-2 family contains a variable number of PUs (motifs Y and Z2). We present evidence, using band shift experiments, that each PU motif binds DNA gyrase with a different affinity. This suggests that the two families are functionally distinct. |
| |
| Keywords: | |
|
|
