Direct shoot organogenesis from hypocotyl explants of Jatropha curcas L. an important bioenergy feedstock |
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Authors: | Nirmala Sahoo Manikannan Thirunavoukkarasu Priya Ranjan Behera Gargi Bala Satpathy Prasanna Kumar Panda |
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Institution: | Bioresources Engineering Department, Institute of Minerals & Materials Technology, (Council of Scientific & Industrial Research), , Bhubaneswar, 751 013 India |
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Abstract: | Multiple shoots were induced on hypocotyl segments reared from in vitro germinated seedlings of Jatropha curcas L., using Murashige and Skoog (MS) medium supplemented with N6‐benzyl adenine (BA) and kinetin (kin) either alone or in combination with indole‐3‐acetic acid (IAA). The combined treatment of 7.05 μm kin and 1.425 μm IAA resulted in maximum shoot production with an average shoot bud initiation of 12.1 per explant. The regenerated microshoots were transferred to root induction medium containing half‐strength MS salts supplemented with either indole‐3‐butyric acid (IBA) or IAA. Rooting of microshoots was best achieved on half‐strength MS medium supplemented with IBA (9.8 μm ). Rooted plantlets were subsequently acclimatized under green house condition and the plantlets showed 70% survival. |
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Keywords: | benzyl adenine hypocotyls Jatropha curcas
kinetin multiple shoot rooting |
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