Fumonisin B1 alters sphingolipid metabolism and immune function in BALB/c mice: Immunological responses to fumonisin B1 |
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Authors: | Elena A Martinova Dr Alfred H Merrill Jr |
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Institution: | (1) Institute of Nutrition, Moscow, Russia;(2) Department of Biochemistry, Emory University School of Medicine, 4113 Rollins Research Center, 30322 Atlanta, GA, USA |
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Abstract: | Fumonisins have been reported to have diverse effects on animals, including immunosuppression in chickens and feeder calves; therefore, the effects of fumonisin B1 (FB1) on immune function in BALB/c mice was investigated. When administered i.p. with sheep red blood cells (SRBC), 5 to 100 µg of FB1 reduced the number of plaque-forming cells (PFC) produced against SRBC; however, when administered daily, 1 to 50 µg of FB1 caused a 4 to 12-fold increase in the number of PFC after SRBC injection. Therefore, FB1 is not only immunosuppressive; but also, immunostimulatory. To test the possibility that there may have been an immune response to FB1 as an antigen, FB1 was injected into mice and the number of splenic cells forming rosettes on FB1-treated SRBC was determined. There were dose-dependent increases in the antigen-binding cells, with up to 4.9- and 4.6-fold increases, respectively, upon primary and secondary immunization. FB1-binding immunoglobulins could be detected in sera from some treated mice, but this response was not obtained in every experiment. In summary, these results show that FB1 has diverse effects on the immune system, causing both stimulation and suppression of the response to foreign antigens, and apparently inducing an antigenic response to FB1.Abbreviations DMEM
Dulbecco's Modified Eagle's Medium
- FB1
fumonisin B1
- FCS
fetal calf serum
- PBS
phosphate buffered saline
- PFC
plaque forming cells
- RFC
rosette forming cells
- SRBC
sheep red blood cells |
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Keywords: | Fumonisins Fusarium moniliforme Immune system Sphingolipids Sphinganine |
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