Effects of Carbon Concentrations and Carbon to Nitrogen ratios on Sporulation of Two Biological Control Fungi as Determined by Different Culture Methods |
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Authors: | Li Gao Xingzhong Liu |
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Institution: | (1) Key Laboratory of Systematic Mycology and Lichenology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of China;(2) State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, People’s Republic of China; |
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Abstract: | The nematophagous fungus Pochonia chlamydosporia (Clavicipitaceae) and entomopathogenic fungus Beauveria bassiana (Cordycipitaceae) have great potential for biological control. However, a significant barrier to their commercial development
as mycopesticides is the high costs associated with production. Carbon (C) concentration and C to nitrogen ratio (C:N ratio)
greatly affect fungal growth and sporulation. Effects of C concentration and C:N ratio differed when the fungi were cultivated
using two different methods: the conventional (continuous cultivation) method and a novel “two-stage” method. Sporulation
of P.
chlamydosporia (HSY-12-14) was the highest when the media contained 6 g l−1 C and a C:N ratio of 40:1 or 8 g l−1 C and C:N ratios of 20:1 or 40:1 for the conventional method but 8 g l−1 C and a C:N ratio of only 10:1 with the novel “two-stage” method. Sporulation of B. bassiana (IBC1201) was the highest when the media contained 12 g l−1 C and a C:N ratio of 40:1 with the conventional method but only 4 g l−1 C and a C:N ratio of 5:1 with the novel “two-stage” method. In addition, the nutritional requirements as determined by the
conventional method differed for mycelial growth and sporulation. Understanding the effects of nutrition on sporulation can
help programs seeking to use these organisms as biological control agents and is essential for their mass production and commercialization. |
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