Viable bacterial biomass and functional diversity in fresh and marine waters in the Canadian Arctic |
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Authors: | L?Tam P?G?Kevan Email author" target="_blank">J?T?TrevorsEmail author |
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Institution: | (1) Department of Environmental Biology, University of Guelph, N1G 2WL , Ontario, Canada; |
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Abstract: | Bacterial biomass and functional diversity in four marine and four freshwater samples, collected from Resolute Bay, Nunavut, Canada, were studied using fluorescent nucleic-acid staining and sole-carbon-source utilization. Viable microbial counts using the LIVE/DEAD BacLight Viability Kit estimated viable marine bacterial numbers from 0.7 to 1.8᎒6 cells/l, which were lower than viable bacterial numbers in freshwater samples (2.1-9.9᎒6 cells/l) (RCBD-ANOVA). Calculations of the Shannon-Wiener diversity index and average well colour development were based on substrate utilization in ECO-Biolog plates incubated at 4°C and 20°C for 38 and 24 days, respectively. The Shannon-Wiener diversity of the marine water samples was significantly greater ( x H'=2.40ǂ.08, P <0.005; RCBD-ANOVA) than that of freshwater samples ( x H'=1.20ǂ.00, P <0.005; RCBD-ANOVA). Differences in microbial diversity between fresh and marine water samples at 4°C ( x 4°C =2.01) and 20°C (x20°C =2.31) were also detected by RCBD-ANOVA analysis. Interactions between water type and incubation temperature were not significant ( F =1.926, F c=5.12). Principal component analysis revealed differences in metabolic substrate utilization patterns and, consequently, the microbial diversity between water types and samples. |
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