Lack of an HSP70 heat shock response in two Antarctic marine invertebrates |
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Authors: | Melody S Clark Keiron P P Fraser Lloyd S Peck |
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Institution: | (1) British Antarctic Survey, Natural Environment Research Council, High Cross, Madingley Road, Cambridge, CB3 0ET, UK |
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Abstract: | Members of the HSP70 gene family comprising the inducible (HSP70) genes and GRP78 (glucose-regulated protein 78 kDa) were
identified in an Antarctic sea star (Odontaster validus) and an Antarctic gammarid (Paraceradocus gibber). These genes were surveyed for expression levels via Q-PCR after an acute 2-hour heat shock experiment in both animals and
a time course assay in O. validus. No significant up-regulation was detected for any of the genes in either of the animals during the acute heat shock. The
time course experiment in O. validus produced slightly different results with an initial down regulation in these genes at 2°C, but no significant up-regulation
of the genes either at 2 or 6°C. Therefore, the classical heat shock response is absent in both species. The data is discussed
in the context of the organisms’ thermal tolerance and the applicability of HSP70 to monitor thermal stress in Antarctic marine
organisms. |
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Keywords: | Antarctic Climate change Biomarker Stress Heat shock proteins |
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