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植物种质的玻璃化超低温保存
引用本文:梁宏,王起华.植物种质的玻璃化超低温保存[J].细胞生物学杂志,2005,27(1):43-45.
作者姓名:梁宏  王起华
作者单位:辽宁师范大学生命科学学院 大连116029 (梁宏),辽宁师范大学生命科学学院 大连116029(王起华)
基金项目:国家自然科学基金(No.30470184,No.30170099)资助项目~~
摘    要:植物种质的玻璃化超低温保存技术已受到广泛重视。玻璃化法主要由装载、玻璃化保护液脱水、降温、复温、洗涤这5个环节构成。目前已对百余种植物进行过玻璃化冻存研究,但主要应用于高等植物,而用该法保存藻类获得成功的报道很少。将玻璃化法用于某些藻类种质的冻存将会有广阔的应用前景。

关 键 词:植物  种质保存  玻璃化  超低温保存
修稿时间:2004年6月7日

The Cryopreservation of Plant Germplasm by Vitrification
Hong Liang,Qi-Hua Wang.The Cryopreservation of Plant Germplasm by Vitrification[J].Chinese Journal of Cell Biology,2005,27(1):43-45.
Authors:Hong Liang  Qi-Hua Wang
Institution:Hong Liang,Qi-Hua Wang*
Abstract:Cryopreservation of plant germplasm by vitrification technique has been appreciated extensivelyto date. Vitrification method consists of 5 steps mainly: loading, dehydration by protective solution of vitrification,cooling, warming and washing (namely unloading). There are hundreds of species of plants being cryopreserved bythis method in the world up to now, but mainly of species of higher plants. However, few successful studies on thecryopreservation of algae germplasm by vitrification technique have been reported so far. Applying vitrificationtechnique to cryopreservation of some species of algae may have significant potential.
Keywords:plant germplasm  cryopreservation  vitrification
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