| 细胞凋亡检测方法探讨 |
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| 引用本文： | 崔巍,唐炳华,王硕仁.细胞凋亡检测方法探讨[J].细胞生物学杂志,2007,29(5):777-782. |
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| 作者姓名： | 崔巍 唐炳华 王硕仁 |
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| 作者单位： | 北京中医药大学 中医内科学教育部重点实验室，北京市普通高校重点实验室(中医内科学)，北京中医药大学基础医学院人体机能系，北京中医药大学，中医内科学教育部重点实验室，北京市普通高校重点实验室(中医内科学)，北京100700，北京100029，北京100700 |
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| 摘 要： | 采用图例法和比较法分析评价细胞凋亡检测方法的应用范围和检测结果的相关性,指出只有选择多种适当的方法进行综合检测,才能对结果做出正确而合理的分析判断。流式细胞术简便、快速,从膜上分子、胞内蛋白到DNA水平全方位多角度分析凋亡,可作为检测细胞凋亡的首选方法。
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| 关 键 词： | 细胞凋亡 检测方法 分析评价 |
| 修稿时间： | 2007-04-06 |
Investigate on the Methodology of Detecting Apoptosis |
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| Wei Cui,Bing-Hua Tang,Shuo-Ren Wang.Investigate on the Methodology of Detecting Apoptosis[J].Chinese Journal of Cell Biology,2007,29(5):777-782. |
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| Authors: | Wei Cui Bing-Hua Tang Shuo-Ren Wang |
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| Institution: | 1.Key Laboratory of Chinese Internal Medicine, Ministry of Education; Beijing Key Laboratory of Chinese Internal Medicine, Beijing University of Chinese Medicine, Beijing 100700, China;2. Department of Human Functions, School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China |
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| Abstract: | By the methods of graphical presentation and comparison, we analyzed and evaluated the relevance between the results got by different apoptosis-detecting techniques and its applicability. Then we found that the more right and reasonable judgments relay on the combination using of different appropriate apoptosis-detecting techniques. Among these methods, flow cytometry has its own advantages. It is more convenient and faster to detect much apoptosis features including membrane moleculars, intracytoplasmic proteins, and DNA fragments. Thus, flow cytometry is recommended to be used as the first choice for apoptosis-detecting. |
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| Keywords: | apoptosis detecting techniques analysis and evaluation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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