褐藻寡糖抗环磷酰胺诱导蚕豆根尖的细胞遗传毒性

采用蚕豆根尖细胞的微核试验和染色体畸变试验方法,测定不同浓度的褐藻寡糖对环磷酰胺(cyclophosphamide,CP)诱导的蚕豆根尖细胞的微核率、有丝分裂指数和染色体畸变率的影响。结果表明:褐藻寡糖能有效抑制环磷酰胺诱导的蚕豆根尖细胞微核的产生,即在一定浓度范围内,微核率随褐藻寡糖处理浓度的降低而减少,但低于一定浓度后反而呈上升趋势;不同浓度的褐藻寡糖均可使蚕豆根尖细胞有丝分裂指数增大;褐藻寡糖还能有效降低蚕豆根尖细胞染色体畸变率。因此,褐藻寡糖对蚕豆根尖细胞具有明显的诱抗活性和调节细胞分裂生长的效应。

Genetic Toxicity of Alginate-derived Oligosaccharide on Vicia faba Root Tip Cells

The effects of different concentrations of alginate-derived oligosaccharide (ADO) on Vicia faba root tip cells were studied. Micronucleus assay and chromosomal aberration assay were used to determine the mitotic index, the micronucleus rate and chromosomal aberration rate of Vivia faba root tip cells induced by cyclophosphamide (CP) under different concentrations of ADO. The result showed that ADO could effectively inhibit the micronucleus rate. During concentration of 10-0.625 g/L, the number of micronucleus of Vivia faba root tip cells were reduced with decrease concentration of ADO, but it was increased when concentration of ADO was lower than 0.625 g/L. The mitotic index was increased, while chromosomal aberration rate were lowed under all concentration of ADO. Turkey test showed all chromosomal aberration rate were significantly different from control (P<0.05). So, ADO has an obvious effect on restraining chromosomal aberration and increasing division of cell of Vivia faba root tip.