| 滋养层细胞侵袭相关基因表达谱分析 |
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| 引用本文： | 范才波,何俊琳,王应雄,刘学庆,陈雪梅,丁裕斌.滋养层细胞侵袭相关基因表达谱分析[J].细胞生物学杂志,2007,29(1):97-102. |
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| 作者姓名： | 范才波 何俊琳 王应雄 刘学庆 陈雪梅 丁裕斌 |
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| 作者单位： | 重庆医科大学公共卫生学院生殖生物学研究室、遗传优生教研室，重庆医科大学公共卫生学院生殖生物学研究室、遗传优生教研室，重庆医科大学公共卫生学院生殖生物学研究室、遗传优生教研室，重庆医科大学公共卫生学院生殖生物学研究室、遗传优生教研室，重庆医科大学公共卫生学院生殖生物学研究室、遗传优生教研室，重庆医科大学公共卫生学院生殖生物学研究室、遗传优生教研室 重庆400016重庆医科大临床检验诊断学省部共建教育部重点实验室，重庆400016，重庆400016，重庆400016，重庆400016，重庆400016，重庆400016 |
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| 基金项目： | 重庆市自然科学基金;重庆市计划生育委员会项目 |
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| 摘 要： | 分离收集正常妊娠第8～12周的细胞滋养层细胞和绒毛外滋养层细胞,提取细胞总RNA,制备cRNA探针并与AffymetrixU133plus2.0基因芯片进行杂交,获得正常细胞滋养层细胞和绒毛外滋养层细胞基因表达谱芯片。经计算机分析共筛选到1318个差异表达基因,其中上调基因813个,下调505个。所有差异表达基因按GeneOntoloty功能分类标准进行了功能检索。为胚胎发育早期绒毛外滋养层细胞侵袭的基因调控机制的研究提供了实验基础。
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| 关 键 词： | 细胞滋养层细胞 绒毛外滋养层细胞 基因芯片 基因表达谱 侵袭 |
| 修稿时间： | 2006-08-072006-10-20 |
Gene Expression Profiles Analysis Related to Invasion of Trophoblasts |
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| Cai-Bo Fan,Jun-Lin He,Ying-Xiong Wang,Xue-Qing Liu,Xue-Mei Chen,Yu-Bin Ding.Gene Expression Profiles Analysis Related to Invasion of Trophoblasts[J].Chinese Journal of Cell Biology,2007,29(1):97-102. |
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| Authors: | Cai-Bo Fan Jun-Lin He Ying-Xiong Wang Xue-Qing Liu Xue-Mei Chen Yu-Bin Ding |
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| Institution: | 1.Laboratory of Reproductive Biology, Department of Genetics, School of Public Health; 2.Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing 400016, China |
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| Abstract: | Cytotrophoblast cells(CTB)and extravillous trophoblast cells(EVT)from 8 week to 12 week normal villous and deciduas were separated and collected.The total RNA of CTB and EVT were extracted respectively.The CTB and EVT gene chip hybridization and gene expression profiles data extraction were performed in Shanghai Biochip Limited Company.The differential expression gene between CTB and EVT were analyzed by computer.We found 1 318 differential expression genes(813 genes up-regulated and 505 genes down-regulated respectively)and queried their biological functions and classified categories according to the standard of Gene Ontoloty.This study would help us to reveal the mechanism of gene network regulation in EVT invasion in early embryo development. |
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| Keywords: | cytotrophoblast cells extravillous trophoblast cells gene chip gene expression profiles invasion |
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