ATP对中性粒细胞H2O2产生双重作用机制的研究

本文通过高压液相法测定ATP的代谢，探讨其对中性粒细胞H2O2产生双重作用的机制。结果显示，ATP本身不能激活中性粒细胞产生H

THE MECHANISMS OF THE BIPHASIC EFFECTS OF ATP ON H2O2 PRODUCTION IN HUMAN NEUTROPHILS

Metabolism of ATP was studied by HPLC assay in order to elucidate the mechanism of the biphasic effects of ATP on H2O2 production by fMLP(formyl-methionyl-leucyl-phenylalanine)-stimulated neutrophils. Results show that ATP was found to be ineffective per se on H2O2 production as a stimulus, but there were biphasic effects on fMLP-stimulated neutrophils. ATP and neutrophils were incubated for 2 min, H2O2 production was increased markedly. With the prolong of time at 5 min, H2O2 production was changed into inhibition. This change is coincided with metabolisn of ATP in time course. ATP starting from 2 min was changed into ADP and AMP, which was breakdowned to adenosine. The inhibition of ATP on neutrophils was antagonized by CGS15943, an antagonist of adenosine. It was indicated that the biphasic effects of ATP, from stimulation to inhibition,was related to metabolism of ATP to adenosine. In other words, the stimulation was performed by P2ureceptor, while the inhibition was performed by adenosine A2 receptor.