浙江地区汉族人群Toll样受体2基因Arg753Gln多态性和肺结核病相关性

应用聚合酶链反应-序列特异性引物方法(polymerase chain reaction with sequence specific primer,PCR-SSP),研究浙江地区汉族人群中Toll样受体2(Toll-like receptor2,TLR2)Arg753Gln(G2408A)单核苷酸多态性(single nucleotide polymorphism,SNP)分布及其与肺结核病的易感性的关系。分析了170名肺结核病患者和199名正常献血者TLR2基因Arg753Gln位点的基因型分布频率。结果表明,在170名肺结核病患者和199名正常献血者中,TLR2 Arg753Gln位点G/G基因型频率分别为58.23%和84.2%,G/A基因型频率分别为41.77%和15.8%,两种基因型在两组中相比较,差异显著,P<0.001。两组人群中均未发现有A/A基因型存在。TLR2基因Arg753Gln位点在浙江地区汉族人群中有其独特的分布规律,这个位点的多态性分布对肺结核病的发展有潜在的危险影响。

The Arg753Gln Polymorphism of the Human Toll-like Receptor 2 Gene and Its Association with Tuberculosis Disease in Zhejiang Han Population

Toll-like receptor 2 (TLR2) plays a critical role in immune response to mycobacteria. In order to know the TLR2 Arg753Gln single nucleotide polymorphism (SNP) and the relationship with the developing of tuberculosis in Zhejiang Han population, we collected the blood samples from 170 TB patients compared with 199 ethnically and age-matched healthy blood donors. Polymerase chain reaction with sequence specific primer method (PCR-SSP) was applied to detect the TLR2 Arg753Gln polymorphism. The G/G genotype of Arg753Gln polymor-phism was observed in 58.23% and 84.2% in TB patients and the controls, respectively. And the G/A genotype in the cases was much greater, 41.77%, compared with 15.8% in the controls. Statistically significant difference was found between the two groups, P<0.001. Moreover, no one in either group was homozygous for the mutation (A/ A). The present data suggests that the polymorphism of the TLR2 gene in Zhejiang Han population has its specific distribution trait. And it influences the risk of developing tuberculosis.