| Cdc42和球形肌动蛋白在卵母细胞胞质分裂中的定位分析 |
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| 引用本文： | 程大也,梁彬,李丰.Cdc42和球形肌动蛋白在卵母细胞胞质分裂中的定位分析[J].细胞生物学杂志,2007,29(3):415-419. |
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| 作者姓名： | 程大也 梁彬 李丰 |
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| 作者单位： | 中国医科大学细胞生物学卫生部重点实验室，中国医科大学细胞生物学卫生部重点实验室，中国医科大学细胞生物学卫生部重点实验室 沈阳110001 中国医科大学附属第一医院输血科，沈阳110001，沈阳110001 中国医科大学高等职业技术学院，沈阳110001，沈阳110001 |
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| 基金项目： | 国家自然科学基金;高等学校博士学科点专项科研项目;教育部留学回国人员科研启动基金 |
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| 摘 要： | 研究活性Cdc42与球形肌动蛋白(G-actin)在爪蟾卵母细胞胞质分裂中的定位关系。分别用GFP-wGBDmRNA与罗丹明-594-微管蛋白、Alexa-488-球形肌动蛋白与罗丹明-594-微管蛋白、GFP-wGBDmRNA与Alexa-594-球形肌动蛋白共同显微注射爪蟾卵母细胞。利用共聚焦显微镜,时间延迟摄影方法,分别观察活体卵母细胞中活性Cdc42、球形肌动蛋白在胞质分裂过程中的定位,以及活性Cdc42与球形肌动蛋白在胞质分裂中的定位关系。在卵母细胞胞质分裂中,活性Cdc42与球形肌动蛋白存在空间上共定位现象,并且在时相上具有一致性。结果提示活性Cdc42和球形肌动蛋白在卵母细胞胞质分裂过程中密切相关。
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| 关 键 词： | 球形肌动蛋白 卵母细胞 胞质分裂 |
| 修稿时间： | 2007-01-172007-05-08 |
Colocalization of Cdc42 and G-actin during Xenopus Oocyte Cytokinesis |
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| Da-Ye Cheng,Bin Liang,Feng Li.Colocalization of Cdc42 and G-actin during Xenopus Oocyte Cytokinesis[J].Chinese Journal of Cell Biology,2007,29(3):415-419. |
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| Authors: | Da-Ye Cheng Bin Liang Feng Li |
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| Institution: | 1Key Laboratory of Cell Biology of Ministry of Public Health of China, China Medical University, Shenyang 110001, China; 2Transfusion Department, First Affiliated Hospital, China Medical University, Shenyang 110001, China; 3Advanced Technical College, China Medical University, Shenyang 110001, China |
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| Abstract: | Study the colocalization of active Cdc42 and G-actin during Xenopus oocyte cytokinesis. GFP-wGBD mRNA and rhodamine-594-tubulin, alexa-488-G-actin and rhodamine-594-tubulin, GFP-wGBD mRNA and alexa-594-G-actin were microinjected into Xenopus oocytes respectively. Employ confocal microscopy and time-lapse experiments to observe localization of active Cdc42 and G-actin as well as colocalization of active Cdc42 and G-actin during oocyte cytokinesis. Results showed that active Cdc42 colocalized with G-actin spatially and temporally, and indicated active Cdc42 might tightly correlated with G-actin during Xenopus oocyte division processs. |
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| Keywords: | Cdc42 |
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