| 海胆黄多糖SEP对S180的体内抑制作用 |
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| 引用本文： | 高宇,虞剑,李冰,康迪,王慧,窦洁,周伟东,奚涛,周长林.海胆黄多糖SEP对S180的体内抑制作用[J].细胞生物学杂志,2011(9). |
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| 作者姓名： | 高宇 虞剑 李冰 康迪 王慧 窦洁 周伟东 奚涛 周长林 |
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| 作者单位： | 中国药科大学生命科学与技术学院; |
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| 基金项目： | “重大新药创制”科技重大专项(No.2009ZX09103-655); 国家基础科学人才培养基金(No.J0630858)资助项目~~ |
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| 摘 要： | 研究海胆黄多糖SEP对S180肉瘤的抑制作用及初步机制。MTT法检测SEP对体外培养的S180细胞生长的抑制作用;建立小鼠S180肉瘤模型观察SEP抗肿瘤活性;检测SEP协同ConA/LPS刺激小鼠脾淋巴细胞增殖作用;同时,考察SEP对NK细胞和杀伤性T淋巴细胞(cytotoxic T lym-phocyte,CTL)活性的影响;碳粒廓清检测SEP对小鼠单核巨噬细胞吞噬功能的影响。研究表明,海胆黄多糖SEP高中低剂量(16、8、4 mg/kg)显著抑制小鼠180实体瘤生长,增加小鼠脾指数和胸腺指数,协同ConA/LPS刺激小鼠脾淋巴细胞增殖,提高小鼠NK细胞和CTL活性,增强小鼠单核巨噬细胞的吞噬功能,通过免疫调节提高小鼠免疫功能达到抑制S180作用。
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| 关 键 词： | 海胆黄多糖SEP 抗肿瘤 免疫调节 NK细胞和CTL活性 |
Antitumor Activity of the Polysaccharride from the Eggs of Strongylocentrotus Nudus(SEP) on S180 in vivo |
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| Gao Yu,Yu Jian,Li Bing,Kang Di,Wang Hui,Dou Jie,Zhou Weidong,Xi Tao,Zhou Changlin.Antitumor Activity of the Polysaccharride from the Eggs of Strongylocentrotus Nudus(SEP) on S180 in vivo[J].Chinese Journal of Cell Biology,2011(9). |
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| Authors: | Gao Yu Yu Jian Li Bing Kang Di Wang Hui Dou Jie Zhou Weidong Xi Tao Zhou Changlin |
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| Institution: | Gao Yu,Yu Jian,Li Bing,Kang Di,Wang Hui,Dou Jie,Zhou Weidong,Xi Tao~*,Zhou Changlin~* (School of Life Science and Technology,China Pharmaceutical University,Nanjing 210009,China) |
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| Abstract: | This research is about the antitumor activity of the polysaccharide from the eggs of Strongylocentrotus Nudus(SEP) on S180 in vivo.MTT assay detected the inhibition effect of SEP on S180 in vitro.Model of S180-bearing mice was built to study the antitumor activity of SEP in vivo.Synergism effect of SEP on spleen lymphocyte proliferation induced by ConA/LPS.Besides,influences of SEP on NK cells and CTL activity were assayed. Carbon expurgation method was used to observe the function of mice mononuclear macro... |
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| Keywords: | SEP(eggs of Strongylocentrotus Nudus) antitumor immunomodulation NK cells and CTL activity |
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