| IL-8对肺腺癌A549细胞迁移的影响及其机制探讨 |
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| 引用本文： | 杨同宁,于超,李佳佳,朱冰,唐云乔,江跃全.IL-8对肺腺癌A549细胞迁移的影响及其机制探讨[J].细胞生物学杂志,2011(10). |
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| 作者姓名： | 杨同宁 于超 李佳佳 朱冰 唐云乔 江跃全 |
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| 作者单位： | 重庆医科大学第二附属医院胸心外科;重庆医科大学生命科学研究院;重庆市肿瘤研究所; |
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| 基金项目： | 重庆市自然科学重点项目(No.2009BA5083); 重庆医科大学重点基金(No.XBED200806)资助项目~~ |
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| 摘 要： | 探讨IL-8对肺腺癌A549细胞迁移的影响及其可能机制。用MTT法选择了合适的IL-8使用浓度。分别用划痕试验及Transwell试验证明了IL-8可以促进肺腺癌A549细胞的迁移。Westernblot结果表明:(1)IL-8可以促进MMP-2蛋白的表达,而对MMP-9的表达无明显影响;(2)IL-8可促进JNK/SAPK磷酸化蛋白的表达;(3)抑制剂(SP600125)可以阻断IL-8对MMP-2蛋白表达的影响。划痕试验从反面验证了低表达的MMP-2可以抑制A549细胞的迁移。表明IL-8可通过JNK/SAPK信号通路调控MMP-2蛋白的表达,进一步促进肺腺癌A549细胞的迁移。
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| 关 键 词： | IL-8 MMP-2 MMP-9 A549细胞 细胞迁移 |
Effect and Mechanism of IL-8 on the Cell Migration in Lung Adenocarcinoma A549 Cells |
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| Yang Tongning,YuChao,Li Jiajia,Zhu Bing,Tang Yunqiao,Jiang Yuequan.Effect and Mechanism of IL-8 on the Cell Migration in Lung Adenocarcinoma A549 Cells[J].Chinese Journal of Cell Biology,2011(10). |
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| Authors: | Yang Tongning YuChao Li Jiajia Zhu Bing Tang Yunqiao Jiang Yuequan |
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| Institution: | Yang Tongning~1,YuChao~2,Li Jiajia~2,Zhu Bing~1,Tang Yunqiao~1,Jiang Yuequan~(3*) (1 Department of Thoracocardiac Surgery,the Second Affiliated Hospital,Chongqing University of Medical Sciences,Chongqing 400010,China,2 Institute of Life Science,Chongqing Medical University,Chongqing 400016,3 Department of Thoracocardiac Surgery,Chongqing Cancer Hospital & Institute,Chongqing 400030,China) |
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| Abstract: | To investigate the effect and mechanism of IL-8 on the cell migration in lung adenocarcinoma A549 cells,the appropriate concentration of IL-8 treated with cells was chosen by MTT arrays.Wound-healing experiment and Transwell arrays were used to prove that IL-8 can promote the migration of lung adenocarcinoma A549 cells.The results of Western blot showed that:(1)IL-8 can promote the expression of MMP-2 protein,but no significant effect on the expression of MMP-9.(2)IL-8 can promote the expression of p-JNK/SA... |
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| Keywords: | IL-8 MMP-2 MMP-9 A549 cells cell migration |
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