| 葡萄胎中MBD2表达及其相关基因甲基化芯片研究 |
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| 引用本文： | 郭良蕊,何俊琳,陈雪梅,刘学庆,王应雄,丁裕斌.葡萄胎中MBD2表达及其相关基因甲基化芯片研究[J].细胞生物学杂志,2011(3). |
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| 作者姓名： | 郭良蕊 何俊琳 陈雪梅 刘学庆 王应雄 丁裕斌 |
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| 作者单位： | 重庆医科大学公共卫生学院生殖生物学实验室; |
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| 基金项目： | 国家自然科学基金(No.30700898); 教育部博士点基金(No.20070631007); 重庆市科委自然科学基金(CSTC,No.2007BB5286); 出生缺陷与生殖健康重庆市重点实验室课题(No.0801); 重庆医科大学校办课题(No.XBYB2008063)资助项目~~ |
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| 摘 要： | 用Real-time RT-PCR、Western blot和免疫组织化学方法分别检测了去甲基化酶MBD2(methyl-CpG-binding domain 2,MBD2)在完全型葡萄胎(complete hydatidiform mole,CHM)和正常早期妊娠绒毛中的表达,用甲基化DNA免疫沉淀MeDIP(methylated DNA immunoprecipitation)-甲基化芯片分析完全型葡萄胎和正常早期妊娠绒毛中相关基因的甲基化情况,用生物信息学分析筛选了差异甲基化基因并进行功能分类。MBD2的mRNA在完全型葡萄胎中的表达明显高于正常早期妊娠绒毛(P=0.0083),Western blot(P=0.0005)和免疫组织化学(P=0.0091)检测到MBD2蛋白表达与Real-time RT-PCR结果一致。结果显示MBD2在完全型葡萄胎中的表达显著高于正常早期妊娠绒毛组织(P<0.01),与正常早期妊娠绒毛组织相比较,完全型葡萄胎组织中相对有89个基因发生了去甲基化,其中85个基因可被映射到基因组图谱中,MBD2在完全型葡萄胎中的高表达及部分基因的去甲基化可能在完全型葡萄胎的发生中扮演了重...
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| 关 键 词： | 完全型葡萄胎 去甲基化酶 MBD2 甲基化DNA免疫沉淀 |
The Expression of MBD2 and Related Gene Methylation Microarray in Hydatidiform Mole |
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| Liang-Rui Guo,Jun-Lin He,Xue-Mei Chen,Xue-Qing Liu,Ying-Xiong Wang,Yu-Bin Ding.The Expression of MBD2 and Related Gene Methylation Microarray in Hydatidiform Mole[J].Chinese Journal of Cell Biology,2011(3). |
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| Authors: | Liang-Rui Guo Jun-Lin He Xue-Mei Chen Xue-Qing Liu Ying-Xiong Wang Yu-Bin Ding |
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| Institution: | Liang-Rui Guo,Jun-Lin He,Xue-Mei Chen,Xue-Qing Liu,Ying-Xiong Wang,Yu-Bin Ding~* (Laboratory of Reproductive Biology,School of Public Health,Chongqing Medical University,Chongqing 400016,China) |
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| Abstract: | The expression of MBD2(methyl-CpG-binding domain2) in complete hydatidiform mole(CHM) and normal early chorionic villi were examined by Real-time RT-PCR,Western blot and immunohistochemistry. Differential methylation pattern of genes in CHM and normal early chorionic villi were examined by MeDIP-chip (methylated DNA immunoprecipitation-chip),analysised and functional charactered by bioinformatics.The results showed mRNA expression(P=0.0083) of MBD2 was significantly higher in CHM than that in normal chorion... |
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| Keywords: | CHM demethylase MBD2 MeDIP |
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