犊牛前脂肪细胞的原代培养

为了建立犊牛前脂肪细胞原代培养模式，以便深入地研究奶牛脂肪组织增生的生物学特征。选用犊牛小肠网膜，采用原代消化细胞培养法培养出梭形细胞；同时以皮肤组织的成纤维细胞培养作为对照。结果显示：培养出的梭形细胞成分均一，增殖旺盛，分化率高。经形态学动态变化的观察，生长曲线及油红O脂肪染色抽取法测定，证明是功能活跃的前脂肪细胞，并在体外重现了其增殖的全过程。因此，在犊牛小肠网膜中存在着可分化成熟的、生成脂肪的前脂肪细胞。为进一步研究与肥胖、胰岛素抵抗相关的疾病如奶牛酮病、脂肪肝等打下了基础。

Primary Culture of Calf Preadipocyte

To establish a primary calf preadipocyte culture method for better understanding the propertiesof hyperplasia of calf adipose tissue, fibroblast like cells from calf small intestine omentum were cultured andfibroblasts from cattle dermise were also cultured to serve as control. Results shown: The cells from intestineomentum were highly homogeneous, proliferative and have high differentiation rate. Their dynamic morphologicalchanges, growth curve, extracting stained intracytoplasmic lipid with oil red O, all verified their preadipocyte identity.Under controlled conditions, the preadipocytes replayed their hyperplasia process in vitro. Conclusion: In calf omen-tum tissue, there exist preadipocytes that can differentiate into mature adipocytes. Since adipocyte is one kind ofclassic target cells to insulin, this study aid the basis for further probing into obesity and insulin resistant diseasessuch as ketosis and fatty liver in dairy cows.