| 胚胎干细胞研究的新工具:RNA干扰 |
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| 引用本文： | 张艳丽,许丹,洪俊君,王子玉,王锋.胚胎干细胞研究的新工具:RNA干扰[J].细胞生物学杂志,2007,29(3):341-345. |
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| 作者姓名： | 张艳丽 许丹 洪俊君 王子玉 王锋 |
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| 作者单位： | 南京农业大学动物胚胎工程技术中心 南京210095 |
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| 摘 要： | 胚胎干细胞研究是20世纪90年代以来在生物医学领域中最引人注目的热点之一,而新近发展起来的RNA干扰技术,能快速有效地沉默基因表达,将成为胚胎干细胞生物学研究的得力工具。现对RNA干扰的作用机制,以及RNA干扰应用于胚胎干细胞研究的方法与RNA干扰在胚胎干细胞研究领域的进展作一综述,以期为今后这方面的研究提供参考。
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| 关 键 词： | RNA干扰 胚胎干细胞 自我更新 细胞分化 |
| 修稿时间： | 2006-10-312006-12-25 |
A New Tool for Studying Embryonic Stem Cells:RNAi |
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| Yan-Li Zhang,Dan Xu,Jun-Jun Hong,Zi-Yu Wang,Feng Wang.A New Tool for Studying Embryonic Stem Cells:RNAi[J].Chinese Journal of Cell Biology,2007,29(3):341-345. |
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| Authors: | Yan-Li Zhang Dan Xu Jun-Jun Hong Zi-Yu Wang Feng Wang |
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| Institution: | Center of Animal Embryo Engineering and Technology, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | Embryonic stem (ES) cells is one of the most popular topics in biomedical research since 1990s, and the newly developed RNA interference (RNAi) technology can silence the genes of interest quickly and efficiently, which would be a very useful tool to study the biological function of ES cells. Here, we review the mechanism of RNAi and the main methods to produce RNAi in ES cells, as well as the prospects of combining RNAi and ES cell manipulation for both basic research and future therapies. |
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| Keywords: | siRNA |
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