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人生长激素突变体的制备及其对受体亲和力和生物活性的研究
引用本文:李伟,朱丽华,汪恩壁,徐燕萍,姜晓东,郭礼和.人生长激素突变体的制备及其对受体亲和力和生物活性的研究[J].细胞生物学杂志,2003,25(1):52-55.
作者姓名:李伟  朱丽华  汪恩壁  徐燕萍  姜晓东  郭礼和
作者单位:中国科学院上海生命科学研究院生物化学与细胞生物学研究所,中国科学院上海生命科学研究院生物化学与细胞生物学研究所,中国科学院上海生命科学研究院生物化学与细胞生物学研究所,中国科学院上海生命科学研究院生物化学与细胞生物学研究所,中国科学院上海生命科学研究院生物化学与细胞生物学研究所,中国科学院上海生命科学研究院生物化学与细胞生物学研究所 上海 200031,上海 200031,上海 200031,上海 200031,上海 200031,上海 200031
摘    要:本文采用DNA重组技术制备了三种重组人生长激素(recombinant human growthhormone,rhGH)突变体,并测定了其受体亲和力及对去垂体大鼠的促体重增加活力。实验结果显示其受体亲和力大小依次为:rhGH-E174A>rhGH-G120R>rhGH>rhGH-G120T。 rhGH-G120R和rhGH-G120T二种突变体相比rhGH来说,对去垂体大鼠促体重增加生物活性明显降低;rhGH-E174A突变体则完全丧失生物活性。由于rhGH-E174A的受体亲和办高于rhGH,表明它是一种较强的hGH拮抗剂,可能成为一种药物,在临床上用于治疗因hGH分泌过多而引起的疾病(例如巨人症,肢端肥大症等)。

关 键 词:人生长激素突变体  制备  生物活性  拮抗剂  受体亲和力
修稿时间:2002年11月11

PREPARATION OF HUMAN GROWTH HORMONE MUTANTS AND STUDIES ON ITS RECEPTOR BINDING ABILITIES AND BIOLOGICAL ACTIVITIES
LI Wei ZHU Li Hua WANG En Bi XU Yan Ping JIANG Xiao Dong GUO Li He.PREPARATION OF HUMAN GROWTH HORMONE MUTANTS AND STUDIES ON ITS RECEPTOR BINDING ABILITIES AND BIOLOGICAL ACTIVITIES[J].Chinese Journal of Cell Biology,2003,25(1):52-55.
Authors:LI Wei ZHU Li Hua WANG En Bi XU Yan Ping JIANG Xiao Dong GUO Li He
Abstract:Three recombinant human growth hormone hGH mutants, rhGH-G120T, rhGH-E174A and rhGH-G120R,were constructed and expressed in E. coli. . Their receptor binding activities were evaluated with their abilities to inhibit the binding of 125I-hGH to growth hormone receptor on the membrane of pregnant rabbit liver cells. The results showed that the order of their receptor binding activities was:rhGH-E174A>rh-GH-G120R>rhGH>rhGH-G120T. rhGH-G120T and rhGH-G120R had lower biological activities to promote the body weight gain in hypophysectomized rat than rhGH, but rhGH-E174A mutant did not have any biological activity to promote the body weight gain in hypophysectomized rat. Therefore, rhGH-E174A is likely to be a good candidate for hGH antagonist.
Keywords:hGH Site-directed mutagenesis Antagonist    
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