银杏叶提取物对牛主动脉内皮细胞增殖及其机制

研究银杏叶提取物(extract of ginkgo biloba,EGB)对牛主动脉内皮细胞(bovine aortic endothelial cells,BAECs)增殖的影响及其机制。分离培养BAECs,给予EGB刺激,采用噻唑蓝比色法检测细胞增殖改变,用流式细胞仪检测对细胞增殖周期的影响,同时用Western印迹检测细胞内皮型一氧化氮合酶(endothelial nitric oxide synthase,eNOS)表达的变化。结果EGB刺激显著促进BAECs的增殖并呈剂量依赖效应,而一氧化氮合酶抑制剂可显著抑制上述效应。EGB刺激显著促进牛主动脉内皮细胞eNOS的表达,并呈剂量依赖效应。EGB显著促进BAECs增殖,其作用由EGB上调的NO介导。

Extract of Ginkgo Biloba Promotes Bovine Aortic Endothelial Cells Growth

To investigate the proliferating effects of extract of ginkgo biloba (EGB) on bovine aortic endothelial cells (BAECs) and relevant mechanism,BAECs were incubated with EGB,and the effects of EGB on BAECs proliferation were investigated by MTT assay,and cell cycle analysis by flow cytometry. Potential involve-ment of signaling pathways of the effects was explored by using related inhibitors of the signal molecules. And the expression of endothelial nitric oxide synthase (eNOS ) was investigated by Western blot. And the results are that EGB markedly promoted BAECs proliferation. Our results also revealed that the effects were significantly attenuated by inhibitors of eNOS,and EGB markedly stimulated the expression of eNOS. EGB markedly promote endothelial cell proliferation,and the effects are mediated by eNOS.