力竭运动诱导的氧化应激对大鼠红细胞Band3蛋白的影响及其机制探讨

为了探讨力竭运动诱导的氧化应激反应对大鼠红细胞Band3蛋白的影响，该文以大鼠跑步运动为模型，对三种不同运动条件下（静坐组、适度运动组和力竭运动组）大鼠红细胞抗氧化能力和氧化损伤程度进行了检测，并对氧化应激反应诱导的红细胞膜Band3蛋白表达和分布情况及其调控的阴离子通道活性进行了分析。结果表明：力竭运动条件下大鼠红细胞受到严重的氧化应激损伤，红细胞内抗氧化能力下降；导致膜Band3蛋白巯基交联为主的蛋白聚簇化反应及其阴离子转运能力的下降。Band3蛋白的损伤将进一步诱导红细胞携氧和变形能力的下降，成为运动相关疾病的潜在致病因素。

Influence of Exhaustive Exercise Induced Oxidative Stress on Band 3 of Rat Erythrocyte

The aim of the present study was to explore the effect of exhaustive exercise-induced oxidative stress on the Band 3 of rat erythrocytes. Rats were divided into sedentary control （C） and exercise test （ET） groups. The ET group was divided further into exhaustive running exercise （ERE） and moderate running exercise （MRE） subgroups. Oxidative stress-induced erythrocyte injure and antioxidant capacity were analyzed. Erythrocytes from control and running exercise groups were evaluated for the expression of erythrocyte Band 3 through immunoblot and immunofluorescence studies. Besides, the anion-transport activity was investigated. The results showed that red blood cells were subjected to severe oxidative stress under exhaustive exercise conditions. Lipid peroxidation and protein sulfhydryl cross-link based clustering of membrane was found after exhaustive exercise, and exhaustive exercise also led to significant increments in the levels of clustering of erythrocyte Band 3 and the decrease of anion-transport activity. Our results suggested that exhaustive running exercise resulted in elevated oxidative stress in erythrocytes, and this change could be associated with the alterations in the structure and function of erythrocyte Band 3.