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小鼠正常肺细胞和肺癌细胞之间的力学特性比较研究
引用本文:牛庆元,张晶晶,纪珍玲,李建亭,马德顺.小鼠正常肺细胞和肺癌细胞之间的力学特性比较研究[J].细胞生物学杂志,2014(3):308-312.
作者姓名:牛庆元  张晶晶  纪珍玲  李建亭  马德顺
作者单位:沈阳大学生命科学与工程学院,沈阳110044
基金项目:沈阳市引智计划(批准号:D20102101006)资助的课题
摘    要:该实验通过鬼笔环肽染色技术观察并比较了小鼠正常肺细胞和肺癌细胞的微丝差异,利用荧光抗体染色技术测定了小鼠单个正常肺细胞和肺癌细胞的α-SMA蛋白含量变化,以及利用CTFM法测定了小鼠正常肺细胞和肺癌细胞的牵引力变化。结果发现,小鼠肺细胞癌变后,细胞内微丝骨架发生了变化,影响了细胞的形态;α-SMA蛋白含量明显下降并且变得分散:细胞投影面积显著减少,大约减少27%,细胞牵引力也显著减小,均方根值大约减少49%。这说明细胞骨架、细胞的形态、α-SMA蛋白和细胞牵引力均与细胞的癌变过程密切相关。

关 键 词:小鼠肺细胞  小鼠肺癌细胞  细胞微丝  细胞牵引力

Comparative Study on the Mechanical Characteristics of the Mice Lung Cells and Lewis Lung Carcinoma
Niu Qingyuan,Zhang Jingjing,Ji Zhenling,Li Jianting,Ma Deshun.Comparative Study on the Mechanical Characteristics of the Mice Lung Cells and Lewis Lung Carcinoma[J].Chinese Journal of Cell Biology,2014(3):308-312.
Authors:Niu Qingyuan  Zhang Jingjing  Ji Zhenling  Li Jianting  Ma Deshun
Institution:(College of Life Science and Engineering of Shenyang University, Shenyang 110044, China)
Abstract:In this article, we observed and compared the microfilament differences with phalloidin stain- ing technology, determined the α-SMA protein changes of single cell with fluorescent antibody staining technol- ogy, and researched the changes of the cell traction force with CTFM (Cell Traction Force Microscopy) method between the mice lung cells and lewis lung carcinoma. The results showed that the microfilament in lewis lung carcinoma changed, and the cell morphology was also affected, α-SMA protein was significantly decreased and dispersed; The cell projection area was significantly reduced by roughly 27%, and the cell traction force was sig- nificantly reduced (RMS was reduced by roughly 49%) in lewis lung carcinoma. These results suggest that the cell skeleton, cell morphology, α-SMA protein and cell traction force are closely related to the process of can- ceration.
Keywords:mice lung cells  lewis lung carcinoma  microfilament  cell traction force
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