| L-茶氨酸对H2O2致L02细胞损伤的保护作用及其机制研究 |
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| 引用本文： | 李桂兰,抗晶晶,殷志敏.L-茶氨酸对H2O2致L02细胞损伤的保护作用及其机制研究[J].细胞生物学杂志,2014(6):785-790. |
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| 作者姓名： | 李桂兰 抗晶晶 殷志敏 |
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| 作者单位： | [1]山西农业大学动物科技学院,太谷030801 [2]江苏省分子与医学重点实验室南京师范大学生命科学学院,南京210046 |
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| 基金项目： | 国家自然科学基金（批准号：81072433、31071000）资助的课题 |
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| 摘 要： | 研究L-茶氨酸对肝细胞损伤的保护作用及其机制。利用H2O2诱导的LO2肝细胞损伤模型，分别用MTT法检测细胞存活率、测定LDH、流式细胞术检测细胞凋亡率、Western blot法检测Caspase-3和PARP蛋白表达7LBax／Bcl-2比值的变化，评价L-茶氨酸是否能保护H2O2诱导的肝细胞损伤。结果表明，L-茶氨酸能提高H2O2损伤的L02细胞存活率，减少LDH的渗漏，降低肝细胞凋亡，且L-茶氨酸通过抑制caspase-3的激活和PARP的切割及Bax／Bcl-2比值的升高而发挥抗凋亡的作用。L-茶氨酸对肝细胞损伤有一定的治疗和保护作用。
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| 关 键 词： | L-茶氨酸 H2O2 L02细胞 保护作用 |
Protective Effect and the Mechanism of L-theanine Against H2O2 Induced Injury in L02 Cells |
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| Li Guilan,Kang Jingjing,Yin Zhimin.Protective Effect and the Mechanism of L-theanine Against H2O2 Induced Injury in L02 Cells[J].Chinese Journal of Cell Biology,2014(6):785-790. |
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| Authors: | Li Guilan Kang Jingjing Yin Zhimin |
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| Institution: | 1 College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China; 2.Jiangsu Province Key Laboratory for Molecular and Medicine Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210046, China) |
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| Abstract: | The object of this study was to explore the protective effect and the mechanism of L-theanine against hepatocyte injury. The human hepatic L02 cells injured by H2O2 were used as a cell model. The protective effect of L-theanine against H2O2-induced hepatocyte injury was evaluated by MTT determination for cell viability, LDH assay, using flow cytometry for apoptosis rate and Western blot for protein expression of Caspase-3, PARP and the ratio of Bax/Bcl-2. The results showed that L-theanine attenuated the cell viability loss and LDH release induced by H2O2, and prevented L02 cells from H2O2-induced cell apoptosis. In addition, L-theanine mediated the anti-apotosis role by decreasing Caspase-3 activation, PARP cleave and Bax/Bcl-2 ratio. In conclusion, L-theanine helps to protect and cure the hepatocyte injury. |
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| Keywords: | L-theanine H2O2 L02 cells protective effect |
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