| CD34分子的胞外结构域cDNA分离与表达 |
| |
| 引用本文： | 孟磊,刘俊霞,贾海蓉,宋增璇.CD34分子的胞外结构域cDNA分离与表达[J].细胞生物学杂志,2003,25(6):376-380. |
| |
| 作者姓名： | 孟磊 刘俊霞 贾海蓉 宋增璇 |
| |
| 作者单位： | 中国医学科学院中国协和医科大学血液学研究所实验血液学国家重点实验室,天津300020 |
| |
| 基金项目： | 天津市科学技术委员会资助项目 (编号03119611)~~ |
| |
| 摘 要： | CD34分子是一种重要的造血干／祖细胞表面标志，广泛用于造血干／祖细胞的鉴定和分选。由于CD34分子具有受体样结构并能传递细胞同型聚集的信号，故它是一种信号分子，但其在造血系统的天然配体与确切的生理功能至今仍不明确。研究中我们分离了CD34分子的细胞外结构域cDNA，并进行了原核融合表达及纯化。DNA序列分析和竞争结合抑制实验证明我们获得了纯化的CD34分子的细胞外结构域的重组蛋白，可作为CD34可溶性受体，用于进一步研究CD34分子的生理功能。
|
| 关 键 词： | CD34分子 胞外结构域 cDNA 分离 表达 原核表达 造血干／祖细胞 |
| 修稿时间： | 2003年4月17日 |
ISOLATION AND EXPRESSION OF CD34 EXTRACELLULAR DOMAIN |
| |
| MENG Lei,LIU Jun Xia,JIA Hai Rong,SONG Zeng Xuan.ISOLATION AND EXPRESSION OF CD34 EXTRACELLULAR DOMAIN[J].Chinese Journal of Cell Biology,2003,25(6):376-380. |
| |
| Authors: | MENG Lei LIU Jun Xia JIA Hai Rong SONG Zeng Xuan |
| |
| Institution: | MENG Lei LIU Jun Xia JIA Hai Rong SONG Zeng Xuan * |
| |
| Abstract: | CD34 molecule is an important hematopoietic stem/progenitor cell s u r face marker, which has been used for identifying and sorting hematopoietic stem / progenitor cells. It is also a signaling molecule because it has receptor like s tructure and can transduce signal for cell homotypic adhesion. However, the nat ural ligand and exact biological function of the CD34 molecule in hematopoietic system have not been clearly identified yet. In this study ,we isolated the ext racellular domain of the CD34 cDNA and expressed as a fusion protein. The result s from DNA sequencing and specific binding assay demonstrated that we have obtai ned a purified recombinant protein of the extracellular domain of CD34 molecule. It will be very helpful for our further studies on the physiological significan ce of the CD34 molecule. |
| |
| Keywords: | CD34 antigen Hematopoietic stem/progenitor cell Prokaryoti c expression |
| 本文献已被 CNKI 维普 等数据库收录！ |
