利用环形染色体构象捕获技术对Bci11b基因座位在细胞核内空间组织的研究

环形染色体构象捕获（4c）技术实现了在全基因组范围内捕获与4c靶位点发生相互作用的基因座位，因而通过4C相关技术可以进一步研究靶基因座位在细胞核内的空间组织形式。该文以ABclllb基因座位作为4C分析的靶位点，通过优化4C分析的反向巢式PCR扩增条件，实现4C分析PCR的高效扩增：并通过有限克隆筛选与普通测序分析相结合的方法，在全基因组范围内捕获到一些与BcHlb基因座位发生潜在相互作用的基因座位。这些基因座位与靶位点间的相互作用既有发生在相同染色体内的，也有发生在不同染色体之间的。这些基因座位间的相互作用表明了Bclllb基因座位在细胞核内复杂的空间组织形式。

Study on the Spatial Organization of Bclllb Gene Locus in Cell Nucleus with the Circular Chromosome Conformation Capture Techniques

The genome-wide gene loci can be captured by the circular chromosome conformation capture （4C） technique, which potentially interacted with the target gene locus of the 4C assay. The spatial organization of the 4C target gene locus in cell nucleus can be further investigated using the 4C-related techniques. In this article, the Bclllb locus was choosen as the target gene locus for 4C assay. Based on the serials of optimization experiments for the PCR conditions, the nested-reverse PCR was efficiently amplified for 4C assay. Combined with the limited clone screening and the general DNA sequencing techniques, many genome-wide gene loci were captured with the 4C assay, which potentially interacted with the Bclllb gene locus. These interactions between the gene loci and the 4C target gene locus were occurred in inter-chromosome and intra-chromosome. The interactions between these gene loci showed the complex spatial organization of Bclllb locus in cell nucleus.