白菜EST-SSR标记的通用性

EST-SSR是从表达序列标签(expressedsequencetag,EST)中开发的新型简单序列重复(simplesequencerepeat,SSR)标记。根据白菜EST设计了15对SSR引物,对白菜、油菜、玉米、高粱、水稻和茶树等进行了PCR,研究了白菜的EST-SSR标记在不同物种间的通用性。所设计的引物对不同白菜品种、近缘种油菜和远缘种玉米、高粱、水稻和茶树的扩增成功率分别为100%、93.3%、80%、93.3%、93.3%和86.7%。在15对引物中,有11对在远缘种中都有扩增产物,而且一些引物可显示多态性,多态性引物分别占了可扩增引物的33.3%、28.6%、28.6%和61.5%。这些结果表明,白菜EST-SSR引物具有较高的通用性,这对于比较基因组学研究有重要意义。

The Transferability of Chinese Cabbage EST-SSR Markers

EST-SSR is a new kind of SSR markers developed from expressed sequence tags (ESTs). Thetransferability of Chinese cabbage EST-SSR markers to different plant species was studied by designing 15 SSRprimer pairs based on ESTs and using them for PCR to genomic DNA from Chinese cabbage, rapeseeds, rice,sorghum, maize and tea tree. The amplification percentage of designed primers to different varieties of Chinesecabbage, relatively related rapeseeds and distantly related rice, sorghum, maize and tea tree, were 100.0%, 93.3%,80.0%, 93.3%, 93.3% and 86.7%, respectively. 11 of the 15 primer pairs showed the amplification in all distantlyrelated species and the polymorphic primers account for 28.6%, 28.6%, 33.3% and 61.5% of available primers inrice, sorghum, maize and tea tree respectively. These results indicate that the Chinese cabbage EST-SSR marker ishighly transferable to other plant species, being significant to studies on comparative genomics among differentspecies.