| FDA-PI双色荧光分光光度法检测细胞活性变化 |
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| 引用本文： | 祁爱群,钱凯先,邵健忠,严庆丰,施沁清.FDA-PI双色荧光分光光度法检测细胞活性变化[J].细胞生物学杂志,2000,22(1):50-52,F003. |
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| 作者姓名： | 祁爱群 钱凯先 邵健忠 严庆丰 施沁清 |
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| 作者单位： | [1]上海市血液中心,上海 [2]浙江大学生物系,杭州 |
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| 摘 要： | 本文建立了FDA-PI双色荧光分光光度法来推测细胞活性的变化，通过图谱初步地观察，进一步测定荧光测值，可以定性和定量地进行分析，此方法简便易行，直观，为测量细胞活性的变化提供一种良好的新方法。
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| 关 键 词： | 细胞活性 FDA－PI双色荧光分光光度法 测定 |
A FLUOROMETRY METHOD TO DETERMINE CELL VIABIlITY BY DOUBLE STAININC WITH FLUORESCEIN DIACETSTE AND PROPIDIUM IODIDE |
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| QI Ai Qun,QIAN Kai Xian,SHAO Jian Zhong,YAN Qing Feng,SHI Qin Qing.A FLUOROMETRY METHOD TO DETERMINE CELL VIABIlITY BY DOUBLE STAININC WITH FLUORESCEIN DIACETSTE AND PROPIDIUM IODIDE[J].Chinese Journal of Cell Biology,2000,22(1):50-52,F003. |
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| Authors: | QI Ai Qun QIAN Kai Xian SHAO Jian Zhong YAN Qing Feng SHI Qin Qing |
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| Abstract: | We established a new double-staining procedure using fluorescein diacetate and propidium iodide to determine cell viability in cell suspension. By watching the fluorograph and calculating the fluorescence value we can do quantitative and qualitative analysis of the cell viability. The method is simple and viable. |
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| Keywords: | Cell viability Spectrofluorophotometer Fluorescein diacetate(FDA) Propidium iodide(P1) C60 Cell strain S180 |
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