| 树突状细胞与CIK细胞共培养诱生的DCCIK细胞群对肿瘤的杀伤作用 |
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| 引用本文： | 庄捷,曹晖,刘祥麟,张尚权.树突状细胞与CIK细胞共培养诱生的DCCIK细胞群对肿瘤的杀伤作用[J].细胞生物学杂志,2007,29(2):237-240. |
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| 作者姓名： | 庄捷 曹晖 刘祥麟 张尚权 |
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| 作者单位： | 上海交通大学医学院附属仁济医院普外科 上海200001(庄捷，曹晖)，中科英达生物技术有限公司 上海201318(刘祥麟)，中国科学院上海生命科学研究院生物化学与细胞生物学研究所 上海200031(张尚权) |
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| 基金项目： | 上海市科委重大科技攻关计划基金 |
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| 摘 要： | 由树突状细胞(DC)与细胞因子诱导的同源杀伤细胞(CIK)的共培养诱生的细胞群(DCCIK)对肿瘤细胞的细胞毒活性的研究。DCCIK细胞体外杀伤肿瘤靶细胞A549(MTT法),效靶比为10∶1、5∶1时杀伤率分别为61%、52%。DCCIK细胞诱导培养3周后,效靶比为10∶1、5∶1时杀伤率分别为64%和56%。数据亦表明DCCIK细胞对靶细胞的杀伤优于CIK细胞。动物体内实验分荷瘤A549、BEL7404和A375三组,每组分(A)DCCIK 化疗、(B)单用化疗。治疗20天、35天后测量各组肿瘤消失率。结果显示:DCCIK 化疗的抑瘤效果明显好于单纯化疗。提示DCCIK细胞有临床应用前景。
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| 关 键 词： | 树突状细胞 细胞因子诱导的杀伤细胞 树突状细胞诱导的细胞因子杀伤细胞:肿瘤免疫治疗 |
| 修稿时间： | 2006-05-29 |
The Immunotherapeutic Antitumor Effect of Dendritic Cells Co-cultured with Cytokine Induced Killer Cells |
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| Jie Zhuang,Hui Cao,Xiang-Lin Liu,Shang-Quan Zhang.The Immunotherapeutic Antitumor Effect of Dendritic Cells Co-cultured with Cytokine Induced Killer Cells[J].Chinese Journal of Cell Biology,2007,29(2):237-240. |
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| Authors: | Jie Zhuang Hui Cao Xiang-Lin Liu Shang-Quan Zhang |
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| Institution: | General Surgical Department, Renji Hospital School of Medicine, Shanghai Jiao Tong University, Shanghai 200001, China; 1.Shanghai Zhong Ke Biotech Co., Shanghai 201318, China; 2.Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Science, Chinese Academy of Science, Shanghai 200031, China |
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| Abstract: | To investigate the antitumor effects of DCCIK which is from dendritic cells were co-cultured with the cytokine induced killer cells (CIK). MTT assays were used to compare the cytotoxicity between DCCIK and CIK cells in vitro. DCCIK cells showed better antitumor cytotoxic effects than the CIK cells. The anticancer effects of DCCIK combined with chemotherapy or chemotherapy alone which were evaluated by using of these cells to treat the Balb/c nude mice which were implanted the A549 lung cancer, BEL-7404 liver cancer and A375 melenoma cells. Measurement of tumor size after 20 and 35 days of treatment, the result indicated that immuno-therapy of DCCIK cells plus chemotherapy had a better antitumor effect than the chemotherapy alone. And it also suggested the DCCIK cells has a potential clinic value. |
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| Keywords: | CIK cells dendritic cells DCCIK cells antitumor immumotherapy |
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