拟南芥悬浮细胞系的玻璃化法超低温保存

悬浮培养细胞系是植物生理生化研究的好材料之一。为了保持细胞系的遗传稳定性，需要采用超低温保存技术。玻璃化法是一种不用程序降温仪的超低温保存技术。本文报道了从模式植物拟南芥建立悬浮细胞系并对其进行玻璃化法超低温研究。细胞经过合理的预培养处理和保护剂处理，直接投入液氮贮存。复温后的细胞能恢复生长，恢复生长的细胞保持着植株再生能力。国外，拟南芥悬浮细胞系的程序降温法保存和包埋脱水法保存已经报道，玻璃化法保存尚未见报道。

Cryopreservation of Arabidopsis Cell Suspensions by Vitrification

Cell suspensions are valuable materials for plant physiology and biochemistry studies. To keep the genetic stability of plant cell suspensions, the cryopreservation technique is required. Vitrification is a method of cryopreservation without the usage of programmable freezers. In the present paper, cell suspensions from Arabidopsis, a model plant, were established and cryopreserved by vitrification. After suitable preculture and dehydration treatment, cells were quenched into liquid nitrogen directly. Thawed cells were able to recover, and recovered cells were able to regenerate plants. This is the first report of cryopreservation of Arabidopsis cell suspension cells by vitrification.