| 利用芸薹属植物嵌合体对组织与器官细胞层起源的研究 |
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| 引用本文： | 王燕,李俊星,陈利萍.利用芸薹属植物嵌合体对组织与器官细胞层起源的研究[J].细胞生物学杂志,2011(8). |
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| 作者姓名： | 王燕 李俊星 陈利萍 |
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| 作者单位： | 浙江大学园艺系农业部园艺植物生长发育与生物技术重点开放实验室; |
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| 基金项目： | 国家自然科学基金(No.30971993); 浙江省重大科技专项(No.2008C12004-2)资助项目~~ |
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| 摘 要： | 运用两种同时具备表型和分子谱系标记的芸薹属植物(榨菜和紫甘蓝)合成的种间平周嵌合体材料对植物的组织、器官的细胞层谱系进行了追踪分析。研究结果发现:植物的茎、叶、花等器官一般由茎尖分生组织的L1、L2、L3三层谱系细胞共同发育而成,但在不同组织器官中各层的贡献量不同;L1和L2层共同参与了叶缘的发育;不定根由L3层单独发育而成;分子标记分析显示花粉起源于L2层,但有性杂交实验也发现了少量L1层起源的花粉。该文为研究植物组织与器官的细胞层起源提供了新方法。
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| 关 键 词： | 组织器官 细胞层起源 嵌合体 谱系标记 榨菜 紫甘蓝 |
Studies on the Origins of Plant Tissues and Organs by Brassica Chimeras |
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| Wang Yan,Li Junxing,Chen Liping.Studies on the Origins of Plant Tissues and Organs by Brassica Chimeras[J].Chinese Journal of Cell Biology,2011(8). |
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| Authors: | Wang Yan Li Junxing Chen Liping |
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| Institution: | Wang Yan,Li Junxing,Chen Liping~* (Department of Horticulture,Zhejiang University,Key Laboratory of Horticultural Plants Growth,Development and Biotechnology,Agricultural Ministry of China,Hangzhou 310058,China) |
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| Abstract: | Two Brassica interspecific periclinal chimeras between tuber mustard and red cabbage which had both phenotypical and molecular markers were applied to systematically study the cell-layer origins of plant tissue and organs.The results showed that L1,L2 and L3 of SAM engaged in the formation and development of almost plant organs together,but their contributions varied in different organs.Both L1 and L2 participated the formation of leaf margin,while the adventitious root was derived from L3 only.PCR analysis... |
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| Keywords: | tissue and organ origin periclinal chimera lineage marker tuber mustard red cabbage |
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