| Mn-SOD基因导入黄瓜的遗传转化体系 |
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| 引用本文： | 范爱丽,孙艳,管清美,梁东.Mn-SOD基因导入黄瓜的遗传转化体系[J].细胞生物学杂志,2011(8). |
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| 作者姓名： | 范爱丽 孙艳 管清美 梁东 |
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| 作者单位： | 西北农林科技大学园艺学院;广西农业科学院蔬菜研究所; |
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| 基金项目： | 国家自然科学基金(No.40971179)资助项目~~ |
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| 摘 要： | 黄瓜是我国栽培面积最大的喜温蔬菜之一。在生产上,高温干旱等环境胁、迫因子严重危害黄瓜的产量和品质,甚至导致绝收。任安芝等综述了SOD与水分、盐分、低温和大气污染等逆境胁迫之间的关系,认为:各种逆境胁迫引起的活性氧代谢平衡失调、生物膜结构破坏是导致植物遭受逆境伤害的机理之一。通过基因工程提高植物体内抗氧化酶活性及增加抗氧化代谢的水平是增强植物抗逆性的有效
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| 关 键 词： | 黄瓜 Mn-SOD基因 遗传转化 |
Establishment of Genetic Transformation System for Cucumber with Mn-SOD Gene |
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| Fan Aili,Sun Yan,Guan Qingmei,Liang Dong.Establishment of Genetic Transformation System for Cucumber with Mn-SOD Gene[J].Chinese Journal of Cell Biology,2011(8). |
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| Authors: | Fan Aili Sun Yan Guan Qingmei Liang Dong |
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| Institution: | Fan Aili~(1,2),Sun Yan~(1*),Guan Qingmei~1,Liang Dong~1 (1 College of Horticulture,Northwest A&F University,Yangling 712100,China,2 Vegetable Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China) |
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| Abstract: | In this study,several factors affecting transformation rate were studied by Agrobacterium-mediated in Nong cheng No.3 cucumber.These factors included sensitivity of cotyledonary nodes to antibiotics (Kan and Cef),days of pre-culture,concentrations of Agrobacterium tumefaciens,days of co-culture and time of being infected on the optimum culture medium and so on.The high frequency transgenic regeneration system of cucumber was established as follows:cotyledons nodes of Nong cheng No.3 cucumber were pre-cultur... |
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| Keywords: | Cucumber(Cucumis Sativus L ) Mn-SOD gene genetic transformation |
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