| 胚胎大鼠背根神经节细胞的分离培养、纯化及生物学特性的研究 |
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| 引用本文： | 王丽琴,宋学琴,王晓娟,吴淑玉,李春岩.胚胎大鼠背根神经节细胞的分离培养、纯化及生物学特性的研究[J].细胞生物学杂志,2003,25(5):320-323,T003. |
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| 作者姓名： | 王丽琴 宋学琴 王晓娟 吴淑玉 李春岩 |
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| 作者单位： | 河北医科大学第二医院神经内科石家庄050000 |
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| 基金项目： | 本研究为河北省自然科学基金资助项目,项目编号302515 |
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| 摘 要： | 本实验取E15 SD胎鼠的背根神经节，用胰蛋白酶消化分离成单细胞，在NBl培养基中培养，并通过差速贴壁法进行背根神经节神经元(DRGn)的分离纯化，用神经元特异性的烯醇化酶(NSE)鉴定培养的神经元。结果发现DRGn在体外合适条件下可存活3-4周，DRGn纯化培养的纯度达91％左右。DRGn在体外能存活较长时间，可作为神经科学研究的细胞模型。
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| 关 键 词： | 背根神经节 细胞培养 神经元 烯醇化酶 |
PURIFICATION AND CHARACTER OF CULTURED EMBRYONIC DORSAL ROOT GANGLION CELLS |
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| WANG Li Qin SONG Xue Qin WANG Xiao Juan WU Shu Yu LI Chun Yan.PURIFICATION AND CHARACTER OF CULTURED EMBRYONIC DORSAL ROOT GANGLION CELLS[J].Chinese Journal of Cell Biology,2003,25(5):320-323,T003. |
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| Authors: | WANG Li Qin SONG Xue Qin WANG Xiao Juan WU Shu Yu LI Chun Yan |
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| Abstract: | Dorsal root ganglions from E15 Sprague Dawley embryonic rats were digested with trypsin and the cell suspension was cultured in NB1 media. Dorsal root ganglion neuron (DRGn) cells were purified by differential adhesion and identified using neuronal specific enolase (NSE) immunocytochemistry stain. DRGn cultured under suitable conditions maitained 3-4 weeks in vitro. The purification rate of purified DRGn by differential adhesion arrived at 91 % . DRGn cultured in vitro can survive longer time and act as a useful cell model in Neuronoscience research. |
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| Keywords: | Dorsal root ganglion Cell culture Neuronal specicfic enolase |
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