魔芋组织培养与细胞工程

近几年来,魔芋组织培养研究进展较快,其细胞工程领域也取得了一定的研究成果。现对魔芋组织培养过程中外植体取材、愈伤组织诱导与分化的激素应用进行了概述,重点介绍了魔芋离体形态建成的几种模式及其调控机制的研究新进展。有关魔芋种质资源离体保存和突变体的筛选的研究工作已经展开,其遗传转化体系也逐渐完善,外源基因如抗病基因、抗除草剂基因等现已转化成功。最后还对魔芋今后的研究方向进行了讨论,指出了目前存在的主要问题并提出了相应的对策。

Tissue Culture and Cell Engineering in Amorphophallus Blume

In recent years, rapid progress has been achieved in tissue culture of konjac as well as in the field of cell engineering. The present article summarized the achievements including explant selection and hormonal application in callus induction and shoot differentiation in konjac tissue culture. Emphases were laid on the new progress on several morphogenetic modes in konjac in vitro and their regulative mechanisms. Also, researches have been carried out in the field of germplasm conservation and mutant selection in vitro. The genetic transformation system was improved gradually and foreign genes, e.g., disease-resistant gene and herbicide-resistant gene, were successfully transformed to konjac. At last, the paper discussed research direction in the future and pointed out the major problems in current researches. Meanwhile, the author put forward the solutions pertinent to the problems.