| 芥蓝不定芽发生过程的基因表达差异分析 |
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| 引用本文： | 黄科,余小林,叶纨芝,向珣,卢钢,曹家树.芥蓝不定芽发生过程的基因表达差异分析[J].细胞生物学杂志,2007,29(1):153-157. |
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| 作者姓名： | 黄科 余小林 叶纨芝 向珣 卢钢 曹家树 |
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| 作者单位： | 浙江大学蔬菜研究所，浙江大学蔬菜研究所，浙江大学蔬菜研究所，浙江大学蔬菜研究所，浙江大学蔬菜研究所，浙江大学蔬菜研究所 杭州310029福建农林大学农产品品质研究所，福州350002福建省农业科学院蔬菜研究中心，福州350013，杭州310029，杭州310029，杭州310029，杭州310029，杭州310029 |
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| 基金项目： | 国家自然科学基金;浙江省科研项目 |
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| 摘 要： | 采用cDNA-AFLP技术和双向电泳技术对不同培养基上的‘中花’芥蓝(Brassicaoleraceavar.alboglabracv.Zhonghua)下胚轴外植体愈伤组织和不定芽发生的基因表达差异进行研究和分析。结果表明:芥蓝不定芽发生基因差异在RNA水平上有所体现,特异条带主要出现在产生不定芽的外植体上,且主要集中在200～600bp之间;芥蓝不定芽发生的基因表达差异在蛋白质水平有很大差异,发生不定芽的外植体和不发生不定芽的外植体出现了160种蛋白质的差异点,差异蛋白的pI值多在5～7之间,在这个范围内的差异蛋白点达到了122个,占差异蛋白总数的76.25%,差异蛋白的分子量多在40～70kDa之间,在这个范围内的差异蛋白点达到了124个,占差异蛋白总数的77.5%。
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| 关 键 词： | 芥蓝 双向电泳 组织培养 表达差异 |
| 修稿时间： | 2006-05-24 |
Differential Gene Expression Analysis of Chinese Kale during Regeneration by cDNA-AFLP and 2-D Electrophoresis |
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| Ke Huang,Xiao-Lin Yu,Wan-Zhi Ye,Xun Xiang,Gang Lu,Jia-Shu Cao.Differential Gene Expression Analysis of Chinese Kale during Regeneration by cDNA-AFLP and 2-D Electrophoresis[J].Chinese Journal of Cell Biology,2007,29(1):153-157. |
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| Authors: | Ke Huang Xiao-Lin Yu Wan-Zhi Ye Xun Xiang Gang Lu Jia-Shu Cao |
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| Institution: | 1.Institute of Vegetable Sciences, Zhejiang University, Hangzhou 310029, China; 2.Institute of Agricultural Product Quality, Fufian Agricultural and Forestry University, Fuzhou 350002, China; 3.Institute of Vegetable Sciences, Fufian Academy of Agricultural Sciences, Fuzhou 350013, China |
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| Abstract: | The differential expression of Chinese kale during regeneration has been analyzed by cDNA-AFLP and 2-D electrophoresis.The results indicated that the differential gene expression of Chinese kale adventi-tious bud occurred at RNA level,the differential band appeared in explants with adventitious bud occurring mainly in 200-600 bp;and the differential gene expression also occurred at protein level,160 differential proteins have been observed,among which 122(76.25% of total differential proteins)proteins' pI were 5-7.Of the total differential proteins,124(77.5% of total differential proteins)proteins' MW distributed at 40-70 kDa. |
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| Keywords: | cDNA-AFLP |
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