哺乳动物卵母细胞生发泡移植

生发泡(GV)移植是指将GV期卵母细胞的GV移入到去核的受体细胞(GV期卵母细胞、MII期卵母细胞或受精卵)透明带下，经融合形成一个重组卵的过程。GV移植对研究卵母细胞的细胞周期调控、成熟及受精时细胞核与细胞质之间的相互作用非常重要，可用于研究卵母细胞减数分裂异常和与年龄相关变化之间的关系及细胞质衰老与卵母细胞非整倍性之间的关系。现简要介绍了GV移植的基本程序，GV核体与胞质体的融合，重组卵的培养条件，重组卵成熟后的受精、人工激活和胚胎发育能力以及GV移植的意义。

Germinal Vesicle Transfer of Mammalian Oocytes

Germinal vesicle (GV) transfer is a technique that GV in GV oocyte is removed and inserted intothe perivitelline space of the enucleated oocyte (GV oocyte, MII oocyte or fertilized egg), forming a reconstructedoocyte by fusion. GV transfer may be a valuable research procedure that generates cell models to characterize thecytoplasmic-nuclear interplay for cell cycle regulation, maturation, and fertilization in oocytes. It can be used tostudy the relationship between meiotic errors and aged-related changes in the oocytes and between oocytoplasmicageing and oocyte aneuploidy. This paper reviews general procedure of GV transfer, fusion of GV-karyoplast andcytoplast, cultural conditions of the reconstructed oocytes, fertilization, artificial activation and developmental ca-pacity of the matured reconstructed oocytes and significance of GV transfer.