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乙醇及6-DMAP对小鼠卵母细胞孤雌激活的研究
引用本文:兰国成,王子玉,马所峰,苗义良,谭景和.乙醇及6-DMAP对小鼠卵母细胞孤雌激活的研究[J].细胞生物学杂志,2002,24(5):307-309.
作者姓名:兰国成  王子玉  马所峰  苗义良  谭景和
作者单位:山东农业大学动物科技学院,泰安271018
基金项目:国家科技部“973”项目(编号G200016107)资助
摘    要:实验研究了乙醇、6-DMAP以及二者联合使用时对注射hCG后18小时采集的小鼠卵母细胞孤雌激活的效果。结果证明:(1)用5%的乙醇分别作用5和10分钟及10%的乙醇分别作用5和10分钟,小鼠卵母细胞的孤雌激活率分别为41.3%、63.7%、57.9%和85.6%。说明在一定范围内,随着乙醇浓度和作用时间的增加,小鼠卵母细胞孤雌激活率有上升的趋势。(2)用2mM 6-DMAP作用2、4和6小时,小鼠卵母细胞的孤雌激活率分别为 12.0%、25.0%和40.0%。说明随着6-DMAP作用时间的增加,小鼠卵母细胞的孤雌激活率有所升高。(3)用5%乙醇作用5分钟,再用含有2mmol/L 6-DMAP的培养液培养6小时,小鼠卵母细胞的孤雌激活率可达65.5%,明显高于单独使用5%乙醇作用5分钟或单独使用2mmol/L 6-DMAP作用6小时卵母细胞的孤雌激活率。(4)用10%的乙醇作用5分钟,再用含有2mmol/L 6-DMAP的培养液培养6小时,小鼠卵母细胞的孤雌激活率达到100%,远远高于单独使用10%乙醇作用5分钟或单独使用2mmol/L 6-DMAP作用6小时卵母细胞的孤雌激活率。(5)在单独使用乙醇刺激时,激活卵母细胞中直接卵裂(2-细胞)的比率随乙醇作用强度的增加而增加,最高达62.5%;但6-DMAP则抑制激活卵母细胞的直接卵裂,增加二原核卵的比例。

关 键 词:乙醇  6-DMAP  小鼠  卵母细胞  孤雌激活
修稿时间:2002年1月18日

PARTHENOGENETIC ACTIVATION OF MOUSE OOCYTES BY ETHANOL AND 6-DMAP
LAN Guo Cheng WANG Zi Yu MA Suo Feng MIAO Yi Liang TAN Jing He.PARTHENOGENETIC ACTIVATION OF MOUSE OOCYTES BY ETHANOL AND 6-DMAP[J].Chinese Journal of Cell Biology,2002,24(5):307-309.
Authors:LAN Guo Cheng WANG Zi Yu MA Suo Feng MIAO Yi Liang TAN Jing He
Abstract:The parthenogenetic response of mouse oocytes to ethanol,6-DMAP or both treatment was investigated in this study. Mouse cocytes collected from superovulated mice 18 hours after hGC injection were stimulated with ethanol and 6-DMAP alone or in combination. The results are as follows: 1. Activation percentages of mouse oocytes were 41.3,63.7,57.9 and 85.6%,respectively, when cocytes were treated with 5 and 10 % ethanol for 5 and 10 minutes, indication that in this case, with the increase of ethanol concentration and duration, the oocyte activation percentage increased significantly. 2. When cocytes were cultured in CZB medium containing 2mmol/L 6-DMAP for 2, 4and 6 hours, the activation rates were 12,25 and 40 % , respectively. 3. When cocytes were first treated in 5 % ethanol for 5 min and then cultured with 2mmol/L 6-DMAP for 6h, the activation rate(65.5 % )was significantly higher than that obtained by treatment with 5 % ethanol or 6-DMAP alone. 4. When oocytes were cultutred in CZB with 2mmol/L 6-DMAP for 6 h after treated in 10 % ethanol for 5 min, the activation percentage reached 100 % , markedly higher than that achieved by 10 % ethanol alone. 5. When oocytes were treated with ethanol alone, with the increase of ethanol concentration and duration the percentage of 2-cell-type(immediate cleavage) activiated oocytes increased significantly,but culture with 6-DMAP inhibited immediate cleavage while increased formation of two pronculei.
Keywords:Parthenogenetic activation Ethanol 6-DMAP Oocyte Mouse
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